AF Fluorescent Dyes

Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.


Check all the info about AF Fluorescent Dyes in our leaflet!


PRODUCTS

AF Dyes: Fluorescent Dyes for High-Performance Labeling

AF 488 is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 488 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

AF 568 is a bright orange-fluorescent dye that is structurally identical to Alexa Fluor 568. With its high fluorescence quantum yield, photostability and pH insensitivity, it has become one of the most widely used fluorophores in life sciences. AF 568 is perfect for a variety of imaging applications, especially super-resolution microscopy such as STORM.

AF 594 is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 which is commonly used for multiplexing. With stable signal generation and its pH insensitivity, its widely used in flow cytometry and super resolution microscopy such as STORM.

AF 647 is a bright far-red-fluorescent dye that is structurally identical to Alexa Fluor 647. AF 647 dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.

"Our AF fluorescent dyes are structurally identical to Alexa Fluor dyes and therefore display same brightness, photostability and other physical properties. Get identical performance for imaging, flow cytometry, and antibody labeling at a significantly lower price."

Jiří Schimer, PhD

Head of Chemistry

Key Parameters

Explore our AF Fluorescent Dyes and their features

Excellent Performance, Lower Price


AF dyes: Same structural composition and quality as Alexa Fluor™ but at a significantly lower cost.

Complete Compatibility


Work seamlessly in any application where Alexa Fluor dyes are used such as: antibody labeling, high-resolution imaging, or sensitive flow cytometry detection

Wide range of applications


AF Dyes are compatible with standard applications like confocal microscopy or FACS and with the advantage ones such as SIM or STED.

Selection Guide

Name

Structurally the same as

Color

Absorb (nm)

Emit (nm)

AF 488

Alexa Fluor 488

Green

495

519

AF 568

Alexa Fluor 568

Orange

579

603

AF 594

Alexa Fluor 594

Red

590

618

AF 647

Alexa Fluor 647

Far-Red

650

671

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