PBlue: Fluorescent Dye

(DB-411500)

PBlue is a blue fluorescent dye that is structurally identical to Pacific Blue. Its bright fluorescence, high quantum yield, and strong signal at neutral pH make it an excellent choice for flow cytometry, fluorescence microscopy, and biomolecule labeling applications.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 350 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

Package size

Key Parameters: Great brightness and pH stability with lower costs

High photostability and pH stability

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Pacific Blue at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Pacific Blue

PBlue: Pick your modification

Name (click for product information)

Availability

PBlue NHS ester

1–2 days (CZ) / within 7 days (EU)

PBlue Carboxylic Acid

1–2 days (CZ) / within 7 days (EU)

PBlue Amine

1–2 days (CZ) / within 7 days (EU)

PBlue Alkyne

1–2 days (CZ) / within 7 days (EU)

PBlue Azide

1–2 days (CZ) / within 7 days (EU)

PBlue PEG4 Alkyne

1–2 days (CZ) / within 7 days (EU)

PBlue PEG4 Azide

1–2 days (CZ) / within 7 days (EU)

PBlue DBCO

1–2 days (CZ) / within 7 days (EU)

PBlue Maleimide

1–2 days (CZ) / within 7 days (EU)

PBlue PEG5-Biotin

1–2 days (CZ) / within 7 days (EU)

PBlue TCO

1–2 days (CZ) / within 7 days (EU)

Optical Data of PBlue

Name

Color

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Cell Permeability

PBlue

Blue

404

455

46 000

Depends on conjugate and reactive group

PBlue: Absorption and emission spectra of PBlue

Absorption and emission spectra of PBlue:The absorption and emission spectra of PBlue visualized. The absorption peak at 404 nm ensures efficient excitation, while the emission peak at 455 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, PBlue is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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