PBlue Maleimide: Fluorescent Dye

(DB-411510)

PBlue is a blue fluorescent dye that is structurally identical to Pacific Blue. Its bright fluorescence, high quantum yield, and strong signal at neutral pH make it an excellent choice for flow cytometry, fluorescence microscopy, and biomolecule labeling applications.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. PBlue is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.


The attached maleimide makes it a thiol-reactive dye for labeling of protein SH groups, and it can be used to attach PBlue fluorophore to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Chemical structure of PBlue
Chemical structure of PBlue over a fluorescence microscopy image

Package size

Chemical structure of PBlue
Chemical structure of PBlue over a fluorescence microscopy image

Key Parameters: Great brightness and pH stability with lower costs

Maleimide: Attaching PBlue to proteins with cystein residues or other thiolated molecules

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Pacific Blue with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Pacific Blue

Pricing

Cat. no.

Quantity

Price

Lead Time

DB-411510-1MG

1 mg

88 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411510-5MG

5 mg

248 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411510-10MG

10 mg

371 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411510-25MG

25 mg

707 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411510-50MG

50 mg

1194 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411510-100MG

100 mg

1768 EUR

1–2 days (CZ) / within 7 days (EU)

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (95 %+ purity)

Optical Data of PBlue Maleimide

Name

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

PBlue Maleimide

404

455

46 000

PBlue Maleimide: Absorption and emission spectra

Excitation and emission spectra of PBlue
Absorption and emission spectra of PBlue:The absorption and emission spectra of PBlue Maleimide visualized. The absorption peak at 404 nm ensures efficient excitation, while the emission peak at 455 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, PBlue is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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Chemical structure of PBlue over a fluorescence microscopy image

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