• DIANA assay

    Novel detection assay with unique benefits for in vitro diagnostics and drug discovery.

Introducing DIANA assay

DIANA (DNA-linked Inhibitor ANtibody Assay) is a novel ultra-sensitive multi-well plate based protein detection assay, which provides unique benefits in its two main areas of application:

  • High Throughput Screening of small-molecular libraries for inhibitors or ligands of protein targets with ultra-low false-negative and false-positive rates
  • Affinity profiling and quantitative specificity panels 
  • ADME pharmacology testing
  • Quantitative detection of active enzymes, receptors or small molecules from range of clinical samples
  • Ultra-sensitive, requiring tiny amount of sample (e.g. drop of blood)
  • Available as a service or in a format of detection kit 

How it works?

DIANA is a multi-well plate assay, where target protein is selectively captured to the solid carrier and then bound by a unique detection probe consisting of a small-molecule ligand linked to a DNA-oligonucleotide. Subsequently, the probe is detected and quantified by qPCR. For drug discovery, the assay utilizes competition between the probe and the test compounds to measure their binding affinity.

DIANA is straightforward to develop for new targets and easy to implement using standard laboratory equipment.

DIANA for drug discovery

  • Quantitative screening of compounds for interactions with the target of interest (enzyme, receptor, protein-protein interactions)
  • Sensitive hit discovery with ultra-low false positive and false negative rates
  • High throughput (>200,000 compounds per day), pooling of compounds to boost throughput and simplify logistics possible
  • Can be used for high throughput screening, selectivity profiling and lead compound optimization
  • Provided as a service or as a easy-to-use kit to be implemented at  customer’s facility

Extremely high signal-to-noise ratio (Z’>0.9;CV<10%)

Quantitative - inhibition potency determined from a single well

Sensitive hit discovery, ultra-low false-positive and false-negative rate

Robust - no need for recombinant or purified proteins

Highly cost-efficient - very low consumption + compatible with pooling

Available as screening kit or as screening service at our facility

DIANA for diagnostics

  • Detection of clinically relevant enzymes, receptors and small molecules in blood or other clinical samples
  • Ultra-sensitive quantitative detection (femtograms of target) with the broad dynamic range (up to 6 logs)
  • Suitable for both R&D and clinical diagnostics applications
  • Assay available in a kit format which could be used in any standard laboratory setting

Ultra-sensitive (zeptomolar) with broad dynamic range

Very low background – no antibody interference

Highly selective for active form of the target

Requires tiny amounts of sample (<1 ul of human blood is sufficient)

CE IVD certification possible

Available in kit format with easy-to-use protocol or as a service

Target portfolio & pipeline

  • DIANA assay has been already developed and validated for 5 targets in the diagnostic mode and for >10 targets in the screening mode
  • Examples of targets include: Carbonic Anhydrase family, Prostate Specific Membrane Antigene or Insulin receptor
  • Assays for multiple additional targets developed on demand (not shown in our pipeline)
  • We focus on various medically relevant enzymes and receptors (incl. multiple onco-markers), mainly on peptide hormones and their receptors and steroids and their receptors and metabolizing enzymes
  •  On demand development of DIANA assay (both for detection and for screening) for a new target is fast and straightforward
TargetDevelopment statusProducts available
Development initiatedProbe synthesized & testedAssay validated - available for licensingScreening
assay
Detection
assay
EnzymesProstate specific membrane antigen (PSMA/GCPII) YESYES
Glutamate carboxypeptidase III (GCPIII) YES
Carbonic anhydrases panel (11 isoforms) YES
Carbonic anhydrases IX and XII YESYES
Fibroblast activation protein (FAP) YESYES
Influenza Neuraminidase N1 YES
RNA polymerase from Influenza YES
17beta-hydroxysteroid dehydrogenase 1 (17beta-HSD1) YES
Methyl transferases YES
Pan-kinase panel
ReceptorsInsulin receptor YES
Immuno-oncology receptors YES

DIANA Biotech story

DIANA Biotechnologies, s.r.o. was established in 2018 as a spin-off company of the Institute of Organic Chemistry and Biochemistry (IOCB), Academy of Sciences in Prague, aiming to build on its global reputation and a strong track record in drug discovery. We have raised a substantial amount of venture capital funding and were awarded an exclusive global rights for the DIANA technology

Combining in our team a unique set of expertise in medical & organic chemistry, biochemistry and pharmacology we are set to unleash the power of DIANA technology in both areas of clinical diagnostics and drug discovery.  

In our diagnostics unit, we are building a portfolio of detection assays  utilizing the DIANA’s ultra-high sensitivity, allowing the detection of medically relevant targets from a tiny amount of sample.  Our drug discovery unit is providing complex CRO services ranging from assay development, HTS screening of small-molecular libraries and ADME pharmacology testing. Utilizing our collaborations we are also developing some of our own early drug-discovery programs. 

The company is now based in its own research facility in a biotechnology hub in Vestec near Prague and has established collaborations with range of industrial and academic partners.

Products & services

Utilizing mainly the proprietary DIANA Technology, but also our general expertise in medicinal chemistry and biochemistry, DIANA Biotechnologies is offering a range of products and services in diagnostics (both for research use and clinical applications) and drug discovery (high throughput screening, affinity measurements and selectivity profiling, assay development, lead compound optimization, ADME pharmacology).

Please check the links above for details and do not hesitate to contact us to discuss your project needs.

News and upcoming presentations

Meet DIANA at ECBS/LS-EuChemS in Madrid

April 3, 2019

We are presenting the DIANA platform at 6th European Chemical Biology Symposium and the meeting of the EuChemS Division “Chemistry in Life Sciences” in Madrid at 3rd-5th April 2019. Meet us there to learn about DIANA’s potential in HTS screening and affinity profiling of Carbonic anhydrase inhibitors and discuss partnership opportunities.

Read more …

Meet DIANA at SLAS 2019 in Washington DC

February 4, 2019

We are presenting DIANA at SLAS 2019 in Washington DC 4th-6th February 2019. Meet us there to learn about DIANA’s potential in HTS screening and affinity profiling and discuss partnership opportunities.

Read more …

DIANA used to identify inhibitors of influenza neuraminidase

December 20, 2018

The team of scientists at IOCB successfully used DIANA technology to screen for inhibitors of influenza neuraminidase.  Their study has been published in Biochemical Journal. Kožíšek et al. (2019): DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors.

Read more …

Meet DIANA at ELRIG Drug discovery 2018 in London

October 9, 2018

We are presenting DIANA at 2018 ELRIG Drug Discovery meeting in London 9th-10th October 2019. Meet us there to learn about DIANA’s potential in HTS screening and affinity profiling and discuss partnership opportunities in drug discovery.

Read more …

DIANA-based research papers

DIANA for drug discovery – High Throughput Screening leading to rapid identification of novel leads.

Methodology paper in preparation

Kožíšek et al. (2019): DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors.

Read more …

Navratil et al. (2017): DIANA for sensitive and selective enzyme detection and inhibitor screening. Nucleic Acids research.

Read more …

Media coverage

Ekonom

25. 4. 2019
Biochemik Václav Navrátil vyvinul metodu, která by mohla odhalit rakovinu nebo třeba Alzheimerovu chorobu v dřívějších stadiích. Vedle diagnostiky chorob najde uplatnění při vývoji léků.

EURO Business

7. 2. 2019
Bývalí uhlobaroni chtějí dát půl miliardy do biotechnologií. Je to vášeň, říká šéf BPD Brůžek

Czech TV, Události komentáře

18. 7. 2018
Příběh – Václav Navrátil. Vynalezl metodu pro tisíckrát citlivější diagnostiku nemocí i nových léků.

Aktuality AV ČR

17. 7. 2018
Start-up ze studentského projektu získal na 80 milionů korun. Vznikla nová start-up společnost DIANA Biotechnologies

Job vacancies - join us!

We are constantly looking for new colleagues to join our growing firm. If you have an education/expertise in pharmacology, molecular biology, biochemistry or medicinal chemistry, if you are interested in working on exciting projects in the areas of clinical diagnostics or drug discovery and if you are willing to work in a fast paced start-up environment, we would love to hear from you.
Please send you CV with a cover letter to jobs@dianabiotech.com

We are currently specifically looking to fill the following vacancies based in Vestec near Prague, Czech republic:

  • Postdoc or PhD in biochemistry, molecular biology or medicinal chemistry (junior applications may be also considered based on the project demand).
  • Experience in drug discovery or clinical diagnostics is a plus
  • Smart, analytical, strong work ethics, easy to work with, skills and motivation to contribute to our R&D projects

Contacts

DIANA Biotechnologies s.r.o.
Nad Safinou II 366
252 50 Vestec
Czech Republic