Pro všechny zájemce o testování na COVID – 19 pomocí našich testů jsme spustili nové webové stránky, kde naleznete veškeré potřebné informace

  • COVID-19 diagnostics

    RNA isolation and ultra-sensitive RT-PCR kits (CE IVD) for SARS-CoV-2 diagnostics. Optimized for high-throughput processing on Agilent Bravo.


Naše PCR testy na COVID-19 ze slin DBdirect kombinují výhody jednoduchého samoodběru a vysoké citlivosti PCR testů (která je výrazně vyšší než u testů antigenních). 

Jedná se však o testy, které je nutno vyhodnotit v jedné z certifikovaných diagnostických laboratoří, kterým testy dodáváme. Naše firma je vývojářem a výrobcem testů, samotné testování pro zákazníky však neprovádíme. V případě zájmu o testování se proto prosím obraťte na jednoho z našich diagnostických partnerů. Ti Vám také poskytnou informace o cenách a další detaily k postupu testování.

Naše PCR testy ze slin veřejnosti a firmám (školám) nabízí již 8 laboratorních řetězců v ČR a 1 na Slovensku (některé mají více odběrových míst a nabízí i možnost svozu vzorků), postupně plánujeme jejich počet dále rozšiřovat:

Laboratoře PREVEDIG (Praha) 

Laboratoře AGELLAB (Morava)  

GHC Genetics (Praha)  

GeneCore BIOCEV (Vestec u Prahy)

  • COVID-19 – GeneCore
  • Laboratoře ve Vestci u Prahy
  • Nabídka pro testování firem a škol 

Hola Biolabs (Brno)  

  • PCR test Brno
  • 2 odběrová místa: Poliklinika Starý Lískovec, Parkoviště CK Victoria – Heršpická 758/13
  • COVID info linka 703 621 191
  • Nabídka pro testování firem

AGEL SK (Zvolen, Slovensko)

Elphogene (Praha)  

VIDIA Diagnostika (Praha)  

Zdravotní ústav Ostrava  

DB-1225 Saliva Collection Set

DIANA COVID-19 diagnostic products

DIANA Biotechnologies has developed series of ultra-sensitive PCR-based diagnostics to fight the COVID-19 epidemic. These are optimized to be run in diagnostic labs in high-throughput format. Our COVID-19 tests are currently in use at 12 major diagnostic centers in the Czech republic and also being exported abroad. We have already sold more than 900 thousands COVID-19 tests, covering 20-25% of Czech testing capacity.

In January 2021 we have introduced to the market our novel technology DBdirect, allowing PCR detection directly from clinical samples (both nasopharyngeal swabs and saliva) without the need of prior isolation of RNA. DBdirect based saliva tests can in our view revolutionize the COVID-19 testing, combining ultra-sensitive detection with the easy self-collection of the samples. These tests are suitable both for clinical diagnostics (CE IVD mark) and preventive screening programs.

  • High efficacy of RNA isolation
  • Reproducible and applicable to a range of biological samples, such as pharyngeal and nasal swabs or saliva
  • Efficacy of COVID-19 RNA isolation is tested for each lot
  • In-house production from basic chemicals, sustainable large scale production 
  • Two versions of the kit currently available:
    • Manual isolation kit (DB-1205)
    • Automated RNA isolation kit for Agilent Bravo (DB-1206, reagents provided in multi-well plate format, includes all the plastic required)
  • Meets all WHO recommendations
  • Multiplex detection of 2 viral genes and 1 RNA isolation control
    • Unique set of primers optimized for ultra-high sensitivity and selectivity 
  • High sensitivity of COVID-19 RNA detection
    • Capable of detecting <10 molecules of viral RNA
    • Higher sensitivity over most commercially available kits
  • Universal: can be used with most multi-well-plate real-time PCR cyclers (96-well or 384-well format)
  • Highly cost-efficient, sustainable large-scale production 
  • CE IVD marked (DB-1211)

Optimized for high-throughput

  • DIANA COVID-19 RNA isolation is optimized to be run in a multi-well plate format on universal laboratory liquid handling robot Agilent Bravo
  • All the necessary protocols and key setup features provided in initial installation package (DB-1214)
  • Single 96-well plate installation can process up to 1500 samples per day

Several other advanced COVID-19 diagnostic solutions currently under development (detection from saliva, self-collection kits, ultra-sensitive serology tests, multiplex COVID&FLU diagnostic kits,…).

Introducing DIANA technology

DIANA (DNA-linked Inhibitor ANtibody Assay) is a novel ultra-sensitive multi-well plate based protein detection assay, which provides unique benefits in its two main areas of application:

  • High Throughput Screening of small-molecular libraries for inhibitors or ligands of protein targets with ultra-low false-negative and false-positive rates
  • Affinity profiling and quantitative specificity panels 
  • ADME pharmacology testing
  • Quantitative detection of active enzymes, receptors or small molecules from range of clinical samples
  • Ultra-sensitive, requiring tiny amount of sample (e.g. drop of blood)
  • Available as a service or in a format of detection kit 

How it works?

DIANA is a multi-well plate assay, where target protein is selectively captured to the solid carrier and then bound by a unique detection probe consisting of a small-molecule ligand linked to a DNA-oligonucleotide. Subsequently, the probe is detected and quantified by qPCR. For drug discovery, the assay utilizes competition between the probe and the test compounds to measure their binding affinity.

DIANA is straightforward to develop for new targets and easy to implement using standard laboratory equipment.

DIANA for drug discovery

  • Quantitative screening of compounds for interactions with the target of interest (enzyme, receptor, protein-protein interactions)
  • Sensitive hit discovery with ultra-low false positive and false negative rates
  • High throughput (>200,000 compounds per day), pooling of compounds to boost throughput and simplify logistics possible
  • Can be used for high throughput screening, selectivity profiling and lead compound optimization
  • Provided as a service or as a easy-to-use kit to be implemented at  customer’s facility

Extremely high signal-to-noise ratio (Z’>0.9;CV<10%)

Quantitative - inhibition potency determined from a single well

Sensitive hit discovery, ultra-low false-positive and false-negative rate

Robust - no need for recombinant or purified proteins

Highly cost-efficient - very low consumption + compatible with pooling

Available as screening kit or as screening service at our facility

DIANA for diagnostics

  • Detection of clinically relevant enzymes, receptors and small molecules in blood or other clinical samples
  • Ultra-sensitive quantitative detection (femtograms of target) with the broad dynamic range (up to 6 logs)
  • Suitable for both R&D and clinical diagnostics applications
  • Assay available in a kit format which could be used in any standard laboratory setting

Ultra-sensitive (zeptomolar) with broad dynamic range

Very low background – no antibody interference

Highly selective for active form of the target

Requires tiny amounts of sample (<1 ul of human blood is sufficient)

CE IVD certification possible

Available in kit format with easy-to-use protocol or as a service

DIANA technology - Target portfolio & pipeline

  • DIANA assay has been already developed and validated for 5 targets in the diagnostic mode and for >10 targets in the screening mode
  • Examples of targets include: Carbonic Anhydrase family, Prostate Specific Membrane Antigene or Insulin receptor
  • Assays for multiple additional targets developed on demand (not shown in our pipeline)
  • We focus on various medically relevant enzymes and receptors (incl. multiple onco-markers), mainly on peptide hormones and their receptors and steroids and their receptors and metabolizing enzymes
  •  On demand development of DIANA assay (both for detection and for screening) for a new target is fast and straightforward
TargetDevelopment statusProducts available
Development initiatedProbe synthesized & testedAssay validated - available for licensingScreening
EnzymesProstate specific membrane antigen (PSMA/GCPII) YESYES
Glutamate carboxypeptidase III (GCPIII) YES
Carbonic anhydrases panel (11 isoforms) YES
Carbonic anhydrases IX and XII YESYES
Fibroblast activation protein (FAP) YESYES
Influenza Neuraminidase N1 YES
RNA polymerase from Influenza YES
17beta-hydroxysteroid dehydrogenase 1 (17beta-HSD1) YES
Methyl transferases YES
Pan-kinase panel
ReceptorsInsulin receptor YES
Immuno-oncology receptors YES

DIANA Biotechnologies story

DIANA Biotechnologies, s.r.o. was established in 2018 as a spin-off company of the Institute of Organic Chemistry and Biochemistry (IOCB), Academy of Sciences in Prague, aiming to build on its global reputation and a strong track record in drug discovery. We have raised a substantial amount of venture capital funding and were awarded an exclusive global rights for the DIANA technology

Combining in our team a unique set of expertise in medical & organic chemistry, biochemistry and pharmacology we are set to unleash the power of DIANA technology in both areas of clinical diagnostics and drug discovery.  

In our diagnostics unit, we are building a portfolio of detection assays  utilizing the DIANA’s ultra-high sensitivity, allowing the detection of medically relevant targets from a tiny amount of sample.  Our drug discovery unit is providing complex CRO services ranging from assay development, HTS screening of small-molecular libraries and ADME pharmacology testing. Utilizing our collaborations we are also developing some of our own early drug-discovery programs. 

The company is now based in its own research facility in a biotechnology hub in Vestec near Prague and has established collaborations with range of industrial and academic partners.

DIANA technology - Products & services

Utilizing mainly the proprietary DIANA Technology, but also our general expertise in medicinal chemistry and biochemistry, DIANA Biotechnologies is offering a range of products and services in diagnostics (both for research use and clinical applications) and drug discovery (high throughput screening, affinity measurements and selectivity profiling, assay development, lead compound optimization, ADME pharmacology).

Please check the links above for details and do not hesitate to contact us to discuss your project needs.

Media coverage

13. 10. 2021
Místo desítek lidí v laboratoři jen pár přístrojů. Ty zvládnou během dne vyhodnotit až deset tisíc vzorků. Tak funguje automatizované vyhodnocování PCR testů na covid ze slin v jedné z českých společností, která své technologie nabízí do dalších laboratoří. Výsledky testů jsou k dispozici ještě v den odběru.

24. 9. 2021
„V naší laboratoři zaznamenáváme zvýšený zájem o PCR testování zejména ze strany firem. Pro ně nabízíme pravidelné testování jednou týdně ultracitlivými a cenově dostupnými PCR testy ze slin,“ řekl Právu Václav Navrátil, vědecký ředitel Diana Biotechnologies

Počty nových covid-19 případů rostou. Avšak pravidelné PCR testování zabraňuje šíření viru ve firmách.

17. 9. 2021
Z posledních dat je zřejmé, že epidemie opět nabývá na síle, neboť podíl pozitivních případů se za poslední 2 měsíce se trojnásobně zvýšil.


27. 8. 2021

Press releases

Počty nových covid-19 případů rostou. Avšak pravidelné PCR testování zabraňuje šíření viru ve firmách.

September 17, 2021

Praha, 16. září 2021 Stáhnout jako PDF. DIANA Lab, laboratoř s nejpokročilejší automatizací a nejvyšší testovací kapacitou v Česku, testuje jak běžné zájemce o test, tak nabízí pravidelné testování pro firmy. Z posledních dat je zřejmé, že epidemie ...

Read more …

Denně až 10 000 PCR testů na jednom místě. Čeští vědci otevírají nejmodernější laboratoř na diagnostiku COVID-19

August 26, 2021

Praha, 24. srpna 2021Stáhnout jako PDF. Česká inovativní společnost DIANA Biotechnologies na Praze 4 otevírá nejmodernější  laboratoř DIANA Lab k diagnostice COVID-19. DIANA Lab s kapacitou 10 000 vzorků denně je první laboratoří v Česku specializov...

Read more …

Šetrné PCR testy ze slin na školách úspěšně nahrazují antigenní testy

May 20, 2021

Praha, 20. května 2021                                                                Stáhnout jako PDF. Ultracitlivé neinvazivní PCR testy ze slin od DIANA Biotechnologies využívají již desítky českých škol k pravidelnému testování. …

Read more …

BIOCEV a DIANA Biotechnologies pomáhají firmám chránit zaměstnance před COVID-19 pomocí spolehlivějších testů

April 12, 2021

Vestec, 12. dubna 2021Stáhnout jako PDF.   Biotechnologický ústav AV ČR v centru BIOCEV nabízí ve spolupráci s Přírodovědeckou fakultou UK pravidelné testování na SARS-CoV-2 pro firmy. Vyšetření probíhá ze vzorků slin technologií PCR (přímý průkaz g...

Read more …

DIANA-based research papers

DIANA for drug discovery – High Throughput Screening leading to rapid identification of novel leads.

Methodology paper in preparation

Kožíšek et al. (2019): DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors.

Read more …

Navratil et al. (2017): DIANA for sensitive and selective enzyme detection and inhibitor screening. Nucleic Acids research.

Read more …

Scientific presentations

Diana Biotechnologies ve FinMagu

August 24, 2021

“Firma do přípravy a vybavení laboratoře vkládá desítky milionů. Ty přitom podle podnikatelských plánů vlastně ještě ani neměla mít. Věří, že je potřeba všechno stihnout do podzimu. Její laboratoř má pomoct státu, kterýsi o pomoc ještě neřekl, a tak tu předpovídají budoucnost, která možná nenastane. DIANA dodala odběratelům v Česku přes dva miliony PCR testů. …

Read more …

Meet DIANA at ECBS/LS-EuChemS in Madrid

April 3, 2019

We are presenting the DIANA platform at 6th European Chemical Biology Symposium and the meeting of the EuChemS Division “Chemistry in Life Sciences” in Madrid at 3rd-5th April 2019. Meet us there to learn about DIANA’s potential in HTS screening and affinity profiling of Carbonic anhydrase inhibitors and discuss partnership opportunities.

Read more …

Meet DIANA at SLAS 2019 in Washington DC

February 4, 2019

We are presenting DIANA at SLAS 2019 in Washington DC 4th-6th February 2019. Meet us there to learn about DIANA’s potential in HTS screening and affinity profiling and discuss partnership opportunities.

Read more …

DIANA used to identify inhibitors of influenza neuraminidase

December 20, 2018

The team of scientists at IOCB successfully used DIANA technology to screen for inhibitors of influenza neuraminidase.  Their study has been published in Biochemical Journal. Kožíšek et al. (2019): DNA-linked inhibitor antibody assay (DIANA) as a new method for screening influenza neuraminidase inhibitors.

Read more …


DIANA Biotechnologies s.r.o.
Nad Safinou II 366
252 50 Vestec
Czech Republic