PBlue NHS Ester: Fluorescent Dye

(DB-411502)

PBlue is a blue fluorescent dye that is structurally identical to Pacific Blue. Its bright fluorescence, high quantum yield, and strong signal at neutral pH make it an excellent choice for flow cytometry, fluorescence microscopy, and biomolecule labeling applications.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. PBlue is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

The NHS Ester is an amine-reactive dye: It can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Package size

Key Parameters: Great brightness and pH stability with lower costs

NHS Ester: label amine groups in proteins, peptides or amino-modified oligos

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower cost alternative with protocol compatibility: Same Spectral Properties & Performance – Works exactly like Pacific Blue at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Pacific Blue

Pricing

Cat. no.

Quantity

Price

Lead Time

DB-411502-1MG

1 mg

80 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411502-5MG

5 mg

224 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411502-10MG

10 mg

336 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411502-25MG

25 mg

640 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411502-50MG

50 mg

1080 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411502-100MG

100 mg

1600 EUR

1–2 days (CZ) / within 7 days (EU)

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (80 %+ purity)

Optical Data of PBlue NHS Ester

Name

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Expected mass added after conjugation (g/mol)

PBlue NHS Ester

404

455

46 000

224.1

PBlue NHS Ester: Absorption and emission spectra

Excitation and emission spectra of PBlue
Absorption and emission spectra of PBlue:The absorption and emission spectra of PBlue NHS Ester visualized. The absorption peak at 404 nm ensures efficient excitation, while the emission peak at 455 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, PBlue is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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