DBdirect™ PCR SYBR Mix [Discontinued]

(DB-1271)

This product has been replaced with new series of enhanced DBdirect™ PCR SYBR Mix SuperSens 2.0 and DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG


Introducing DBdirect™ PCR SYBR Mix, a ready-to-use solution containing SYBR® Green I dye for real-time quantification of DNA sequences.


Designed for routine and high-throughput DNA target detection, our unique DBdirect™ technology eliminates the need for DNA extraction, ensuring faster and more streamlined workflows.

Package size

Key Parameters: No need for DNA isolation with faster and more streamlined workflows

Real Time PCR with aptamer hot-start.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Not suitable for single-cell detection. For a single-cell detection try our DBdirect™ PCR SYBR Mix SuperSens.

Product info

Cat. no.

Quantity

Lead Time

DBdirect™ PCR SYBR Mix (DB-1271)

100 rxns

Discontinued

DBdirect™ PCR SYBR Mix (DB-1271)

1000 rxns

Discontinued

DBdirect™ PCR SYBR Mix (DB-1271)

5000 rxns

Discontinued

Details

Key Protein

Hot-start

Content

QC Assays

Taq DNA Polymerase

Aptamer-based

DBdirect™ PCR SYBR Mix (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms

DB-1271 with LBA

DB-1271 PCR Mix

Human Cells

+++

+++

Gram-negative bacteria (E. coli)

++

++

Gram-positive bacteria (Lactobacillus)

++

+++

Gram-positive bacteria (Rhodococcus)

++

+++

Yeast (S. Cerevisiae)

++

++

Non-enveloped viruses (ADV)

+++

+++

Enveloped viruses (HSV-1)

+++

+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

Downloads

Document name

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Let's take your PCR to the next level!

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