DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG: Boosted Accuracy

DBdirect^™ PCR SYBR Mix SuperSens 2.0 UDG

(DB-1316)

Introducing next-generation of DBdirect^™ PCR SYBR Mix SuperSens, a ready-to-use solution containing SYBR® Green I dye for real-time quantification of DNA sequences.

Perfect for routine and high-throughput DNA target detection, this mix features our unique DBdirect™ technology, eliminating the need for DNA extraction.

The 2.0 series of PCR Mixes is exceptionally sensitive and robust. Our mixes detect a single DNA copy. Their stability and user-friendly packaging make them ideal for everyday analysis. All mixes in the 2.0 series are suitable for single-cell detection. Contains UDG (uracil-DNA glycosylase) to prevent carryover contamination from previous PCR reactions.

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Package size

Key Parameters: No need for DNA extraction with unique specificity

Exceptionally robust and sensitive: The 2.0 series of DB PCR Mixes can detect approx. 1 copy of DNA. Stable for at least days at 25°C, at least several weeks at 4°C

Real-time PCR with proprietary, ultra-efficient antibody hot-start

Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order)

UDG variant contains optimal ratio of dTTP and dUTP to maximize robustness without compromising UDG efficiency.

Ambient-temperature shipping: Reduces cold-chain packaging and transport emissions, supporting more sustainable delivery.

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (DB-1316)	100 rxns	57 EUR	1–2 days (CZ) / within 7 days (EU)
DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (DB-1316)	1000 rxns	497 EUR	1–2 days (CZ) / within 7 days (EU)
DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (DB-1316)	5000 rxns (5x1000 rxns)	2113 EUR	1–2 days (CZ) / within 7 days (EU)
DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (DB-1316)	10 000 rxns	Upon request	Upon request

Find Your Distributor

Czechia, Slovakia and Hungary	UK and Ireland	Italy	Germany, Switzerland and Austria	Denmark, Sweden, Finland, Iceland and Norway	Benelux, Spain and Portugal
BioTech	Caltag	VitaSan Labs	Hoelzel Biotech	Techtum	Isogen

Details

Key Protein	Hot-start	Content	QC Assays
DB SuperSens Taq DNA Polymerase	Antibody-based hot-start	DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (2x) PCR Grade Water	Functional test (PCR) E. coli gDNA absence DNase Activity absence

Key Protein

Hot-start

Content

QC Assays

DB SuperSens Taq DNA Polymerase

Antibody-based hot-start

DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

Testimonials

"The DBdirect^™ PCR SYBR Mix SuperSens kit delivers higher efficiency qPCR curves and enhanced sensitivity for detecting low copy numbers of small extrachromosomal circular DNA in tumors, compared to standard PCR master mixes."

Egija Zole, University of Copenhagen, Department of Biology, Ecology and Evolution

Downloads

Document name	Download

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect^™ Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E. coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms	DB-1316 with LBA	DB-1316 PCR Mix
Human Cells	+++	+++
Gram-negative bacteria (E. coli)	+++	+++
Gram-positive bacteria (Lactobacillus)	++	+++
Gram-positive bacteria (Rhodococcus)	+++	++
Yeast (S. cerevisiae)	+++	+
Non-enveloped viruses (ADV)	+++	+++
Enveloped viruses (HSV-1)	+++	+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

Order or request free sample

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