DB PCR Mixes: Robust PCR beyond ideal laboratory conditions

Born in large-scale molecular diagnostics and proven in millions of reactions, our DB PCR Mixes 2.0 bring diagnostic-grade robustness, sensitivity and reproducibility to your research.


Engineered enzymes, proprietary hot-start technology and optimized buffers deliver reliable results, even with challenging samples and complex multiplex assays.


  • Single-component: Ensures maximum simplicity and consistent results
  • DBdirect™ technology: No DNA extraction needed
  • SuperSens technology: Enables superior robustness, sensitivity and specificity.

Our PCR Mixes are available also as Lyo-Ready product with the same final composition!


We created the complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors. Find everything you need in one place. Download it HERE.


DBdirect™ PCR Probe Mix SuperSens 2.0 kit box and tubes
DBdirect™ PCR Probe Mix SuperSens 2.0 kit box and tubes

PRODUCTS

DB PCR Mixes 2.0: Meet our unique solutions DBdirect™ and SuperSens!

DBdirect™ PCR Probe Mix SuperSens 2.0 kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity!

DBdirect™ PCR SYBR Mix SuperSens 2.0 (DB-1310) kit box and tubes

Next generation of DBdirect™ PCR SYBR Mix with no need for DNA extraction enhanced with SuperSens technology for greater accuracy and sensitivity!

DBdirect™ PCR Gel Mix SuperSens 2.0 (DB-1311) kit box and tubes

Next generation of DBdirect™ PCR Gel Mix SuperSens with higher sensitivity and robustness. Direct PCR with no need for DNA extraction enhanced with SuperSens technology for greater accuracy and sensitivity! Containing blue and yellow dyes to monitor migration process during electrophoresis.

DBdirect™ PCR Probe Mix SuperSens 2.0 UDG kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity with UDG!

DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG (DB-1316) kit box and tubes

Next generation of DBdirect™ PCR SYBR Mix with no need for DNA extraction enhanced with SuperSens technology for greater accuracy and sensitivity!

DBdirect™ PCR Gel Mix SuperSens 2.0 UDG (DB-1317) kit box and tubes

Next generation of DBdirect™ PCR Gel Mix SuperSenswith higher sensitivity and robustness. Direct PCR with no need for DNA extraction enhanced containing blue and yellow dyes to monitor migration process during electrophoresis.

DB Lysis Buffer A (DB-1281) bottle

Lysis Buffer suitable for a wide range of samples. Extract your gDNA from human cells or DNA viruses.

DBdirect™ PCR Probe Mix SuperSens 2.0 kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity! Now available in lyo-ready version and enhanced formulation with dedicated primer stabilizing solution for improve primer stability at ambient temperature.

DBdirect™ PCR Probe Mix SuperSens 2.0 kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity! Now available in lyo-ready version and flexible reconstitution using either 2X or 4X buffer, allowing users to select the preferred workflow setup.

DBdirect™ PCR Probe Mix SuperSens 2.0 UDG kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity with UDG! Now available in lyo-ready version and enhanced formulation with dedicated primer stabilizing solution for improve primer stability at ambient temperature.

DBdirect™ PCR Probe Mix SuperSens 2.0 UDG kit box and tubes

Next generation of DBdirect™ PCR Probe Mix SuperSens with no need for DNA extraction. Stable formulation, exceptional robustness and sensitivity with UDG! Now available in lyo-ready version and flexible reconstitution using either 2X or 4X buffer, allowing users to select the preferred workflow setup.

Choose the right variant

One chemistry, multiple formats

The mixes are convenient, single-component formulations designed for practical routine use. Thanks to their exceptional thermal stability, the mixes are suitable for ambient temperature shipment.


All SuperSens 2.0 mixes share the same core enzyme system and optimized buffer chemistry while supporting different detection methods.

1.

Probe Mixes

Designed for hydrolysis probe-based qPCR, including TaqMan® workflows, our Probe Mixes are the choice when maximum sensitivity and specificity count. The SuperSens antibody hot-start suppresses non-specific amplification, so you can reliably detect low-copy targets, down to the single-cell level. With DBdirect™, you can amplify directly from cells, serum or saliva without extraction. Ideal for gene expression, genotyping, pathogen detection and multiplex assays.

2.

SYBR mixes

Our SYBR Mixes come with SYBR Green I already included, giving you a fast and cost-effective way to quantify DNA without designing probes. They are a perfect fit for assay development, primer screening and optimization. Melting-curve analysis confirms product specificity in every run, while SuperSens chemistry keeps background low and sensitivity high, even with single-cell input.

3.

Gel Mixes

When you need a clear yes-or-no answer, our Gel Mixes make endpoint PCR as simple as possible. Tracking dyes and a density additive are already included, so you can load your reaction straight onto an agarose gel. Combined with direct amplification from crude samples, this cuts your workflow to just a few steps. Use them for routine screening, genotyping, colony PCR or preparing products for T/A cloning.

4.

UDG Versions Available

Every format is also available as a UDG version containing dUTP and uracil-DNA glycosylase. Carry-over amplicons from previous reactions are degraded by UDG before the new PCR begins, protecting your results from false positives. This is essential in high-throughput labs and routine testing, giving you the same SuperSens sensitivity and DBdirect™ convenience with an extra layer of reliability.

Key Parameters

Two unique technologies, validated on the samples you actually work with

DBdirect™ Technology

Skip nucleic acid extraction and amplify directly from crude samples. Fewer steps, less hands-on time and lower cost per result.

SuperSens Technology

Our antibody hot-start blocks polymerase activity until the first denaturation step, minimizing background and enabling detection of low-copy targets.

Validated Matrices

Whole blood, blood plasma, blood serum, saliva, swab transport media, mouse tail lysates and cell cultures.

Validated Organisms

Without pretreatment: viruses, Gram-negative bacteria and yeasts. With DB Lysis Buffer A: mammalian cell lines and Gram-positive bacteria.

Various Applications

qPCR from cDNA, genomic DNA and plasmid DNA, genotyping, pathogen detection, and preparation of PCR products for T/A cloning.

Lyo-Ready Formats

Selected mixes are also available as Lyo-Ready versions with the same final composition, ready for lyophilization and ambient-temperature workflows.

"Our unique DBdirect™ technology will speed up your workflow and reduce costs by eliminating the need for NA extraction."

Eva Mlynarčíková

Lead Developer in PCR chemistry

UDG system for carryover contamination control


Are you worried, that the previous run may contaminate the next one? Just pick one of our UDG mixes and the problem is solved. This technology widely used in diagnostic kits is now available for everyone and in every DIANA mix type. The UDG versions of the kit contain optimized ratio of dTTPs and dUTPs along with Uracyl-DNA Glycosylase – enzyme that removes uracyl from the DNA that can’t therefore be used as template. With our UDG mix, any leftover PCR product is taken care of in the beginning of the reaction and you can be sure that what you are measuring is what is meant to be measured. However, if uracyl-containing DNA would interfere with your downstream applications, just pick one of the non-UDG mixes.

Choose the variant that suits you the most

Cat. No.

Name

Ultrasensitive detection

Single cell detection

Long amplicons

TaqMan compatible

SYBR Green I included

Direct gel loading

dUTP included

UDG included

DB-1308

DBdirect™ PCR Probe Mix SuperSens 2.0

✅

✅

✅

✅

❌

❌

❌

❌

DB-1309

DBdirect™ PCR Probe Mix SuperSens 2.0 UDG

✅

✅

✅

✅

❌

❌

✅

✅

DB-1310

DBdirect™ PCR SYBR Mix SuperSens 2.0

✅

✅

✅

❌

✅

❌

❌

❌

DB-1316

DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG

✅

✅

✅

❌

✅

❌

✅

✅

DB-1311

DBdirect™ PCR Gel Mix SuperSens 2.0

✅

✅

✅

❌

❌

✅

❌

❌

DB-1317

DBdirect™ PCR Gel Mix SuperSens 2.0 UDG

✅

✅

✅

❌

❌

✅

✅

✅

Case study: Mouse genotyping in 30 minutes, without DNA extraction

Case study: direct PCR detection of the β2-microglobulin gene from mouse tail tip and hair follicle lysates with DBdirect™ PCR Gel Mix
DBdirect™ PCR Gel Mix efficiently detected the target in both mouse tail tip and hair follicle:Lysates were prepared from a 2 mm mouse tail tip (250 µl DB Lysis Buffer A) or a hair follicle (125 µl DB Lysis Buffer A) by incubating for 30 minutes at 95 °C. 0.1, 1 and 5 µl of lysate were added to DBdirect™ PCR Gel Mix (15–19 µl) and amplified without any DNA purification. PCR products were loaded directly onto a 4% agarose gel. The correct 177 bp product was detected at all lysate volumes. Ladder: Invitrogen Ultra Low Range DNA Ladder.

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Featured Article

Rapid and Sensitive Analysis: Direct PCR from Microorganisms and Cells

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