(DB-1317)
Next generation of DBdirect™ PCR Gel Mix SuperSens 2.0 UDG, a ready-to-use solution featuring blue and yellow dyes for monitoring migration during electrophoresis and an additive for direct loading onto agarose gel.
Our unique DBdirect™ technology, eliminates the need for DNA extraction, ensuring faster, more streamlined workflows.
The 2.0 series of RT-PCR Mixes is exceptionally sensitive and robust. Our mixes can detect work with 1 DNA copy. Its stability and user friendly packaging is ideal for everyday analysis. All mixes in the 2.0 series are suitable for single-cell detection. Contains UDG (uracil-DNA glycosylase to prevent carryover contamination from previous PCR reactions.
Package size
Key Parameters: Gel electrophoresis PCR with unique sensitivity and robustness
Exceptionally robust and sensitive: The 2.0 series of DB PCR Mixes can detect approx. 1 copy of DNA. Stable for at least days at 25°C, at least several weeks at 4°C
End-point PCR with proprietary, ultra-efficient antibody hot-start. Blue and yellow dyes to monitor migration process during electrophoresis.
Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.
Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).
Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).
UDG variant contains optimal ratio of dTTP and dUTP to maximize robustness without compromising UDG efficiency.
Pricing
Cat. no. | Quantity | Price | Lead Time |
|---|---|---|---|
DBdirect™PCR Gel Mix SuperSens 2.0 UDG (DB-1317) | 100 rxns | 57 EUR | In Stock |
DBdirect™PCR Gel Mix SuperSens 2.0 UDG (DB-1317) | 1000 rxns | 497 EUR | In Stock |
DBdirect™PCR Gel Mix SuperSens 2.0 UDG (DB-1317) | 5000 rxns (5x1000 rxns) | 2113 EUR | In Stock |
DBdirect™PCR Gel Mix SuperSens 2.0 UDG (DB-1317) | 10 000 rxns | Upon request | Upon request |
Details
Key Protein | Hot-start | Content | QC Assays |
|---|---|---|---|
Antibody-based hot-start | DBdirect™ PCR Gel Mix SuperSens 2.0 UDG (2x) PCR Grade Water | Functional test (PCR) E. coli gDNA absence DNase Activity absence |
Sensitivity of DBdirect™ PCR mixes across cell types and viruses
Organisms | DB-1317 with LBA | DB-1317 PCR Mix |
|---|---|---|
Human Cells | +++ | +++ |
Gram-negative bacteria (E. coli) | +++ | +++ |
Gram-positive bacteria (Lactobacillus) | ++ | +++ |
Gram-positive bacteria (Rhodococcus) | ++ | ++ |
Yeast (S. Cerevisiae) | +++ | ++ |
Non-enveloped viruses (ADV) | +++ | +++ |
Enveloped viruses (HSV-1) | +++ | +++ |
+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn
Downloads
Document name | Download |
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