(DB-1310)
Introducing next-generation of DBdirect™ PCR SYBR Mix SuperSens, a ready-to-use solution containing SYBR® Green I dye for real-time quantification of DNA sequences.
Perfect for routine and high throughput DNA target detection, this mix features our unique DBdirect™ technology, eliminating the need for DNA extraction.
The 2.0 series of PCR Mixes is exceptionally sensitive and robust. Our mixes can detect work with 1 DNA copy. Its stability and user friendly packaging is ideal for everyday analysis. All mixes in the 2.0 series are suitable for single-cell detection.
Package size
Key Parameters: No need for NA extraction with unique specificity
Exceptionally robust and sensitive: The 2.0 series of DB PCR Mixes can detect approx. 1 copy of DNA.
Real Time PCR with proprietary, ultra-efficient antibody hot-start
Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.
Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).
Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order)
Stable for at least days at 25°C, at least several weeks at 4°C
Pricing
Cat. no. | Quantity | Price | Lead Time |
|---|---|---|---|
DBdirect™ PCR SYBR Mix SuperSens 2.0 (DB-1310) | 100 rxns | 57 EUR | In Stock |
DBdirect™ PCR SYBR Mix SuperSens 2.0 (DB-1310) | 1000 rxns | 497 EUR | In Stock |
DBdirect™ PCR SYBR Mix SuperSens 2.0 (DB-1310) | 5000 rxns (5x1000) | 2113 EUR | In Stock |
DBdirect™ PCR SYBR Mix SuperSens 2.0 (DB-1310) | 10 000 rxns | Upon request | Upon request |
Details
Key Protein | Hot-start | Content | QC Assays |
|---|---|---|---|
Antibody-based hot-start | DBdirect™ PCR SYBR Mix SuperSens 2.0 (2x) PCR Grade Water | Functional test (PCR) E. coli gDNA absence DNase Activity absence |
Testimonials
"The DBdirect™ PCR SYBR Mix SuperSens kit delivers higher efficiency qPCR curves and enhanced sensitivity for detecting low copy numbers of small extrachromosomal circular DNA in tumors, compared to standard PCR master mixes."
Downloads
Document name | Download |
|---|---|
Sensitivity of DBdirect™ PCR mixes across cell types and viruses
Organisms | DB-1310 with LBA | DB-1310 PCR Mix |
|---|---|---|
Human Cells | +++ | +++ |
Gram-negative bacteria (E. coli) | +++ | +++ |
Gram-positive bacteria (Lactobacillus) | ++ | +++ |
Gram-positive bacteria (Rhodococcus) | +++ | ++ |
Yeast (S. Cerevisiae) | +++ | + |
Non-enveloped viruses (ADV) | +++ | +++ |
Enveloped viruses (HSV-1) | +++ | +++ |
+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn
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