DBdirect PCR Probe Mix SuperSens 2.0 UDG: Next-Gen Accuracy

DBdirect^™ PCR Probe Mix SuperSens 2.0 UDG: Exceptional robustness and sensitivity

(DB-1309)

Next generation of the DBdirect^™ PCR Probe Mix SuperSens, ready-to-use solution crafted specifically for TaqMan probes, ideal for routine and high-throughput DNA target detection.

DBdirect^™: Benefit from faster, more streamlined workflows with no need for DNA extraction.

Thanks to its new composition our PCR Probe Mix has an exceptional robustness and sensitivity. Contains UDG (uracil-DNA glycosylase to prevent carryover contamination from previous PCR reactions.

Package size

Key Parameters: No need for DNA isolation with faster and more streamlined workflows

Exceptional robustness and sensitivity: Sensitivity of 1 copy DNA. Stable for at least days at 25°C, at least several weeks at 4°C

Real Time PCR with proprietary, ultra-efficient antibody hot-start. Analyze multiple targets in one reaction.

Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).

UDG variant contains optimal ratio of dTTP and dUTP to maximize robustness without compromising UDG efficiency.

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (DB-1309)	100 rxns	57 EUR	In Stock
DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (DB-1309)	1000 rxns	497 EUR	In Stock
DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (DB-1309)	5000 rxns (5x1000 rxns)	2113 EUR	In Stock
DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (DB-1309)	10 000 rxns	Upon request	Upon request

Details

Key Protein	Hot-start	Content	QC Assays
DB AptaTaq DNA Polymerase	Antibody-based hot-start	DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (2x) PCR Grade Water	Functional test (PCR) E. coli gDNA absence DNase Activity absence

Key Protein

Hot-start

Content

QC Assays

DB AptaTaq DNA Polymerase

Antibody-based hot-start

DBdirect™ PCR Probe Mix SuperSens 2.0 UDG (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

Testimonials

„The DBdirect^™ PCR Probe Mix SuperSens kit enabled direct quantification of Hepatitis B virus DNA from cell culture media without the need for viral DNA purification. This made it feasible to conduct large, dose-dependent compound studies in 96- or 384-well formats, even in media containing 10% fetal bovine serum, without PCR inhibition.“

Jan Weber, Head of virology, IOCB Prague

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms	DB-1309 with LBA	DB-1309 PCR Mix
Human Cells	+++	+++
Gram-negative bacteria (E. coli)	+++	+++
Gram-positive bacteria (Lactobacillus)	+++	+++
Gram-positive bacteria (Rhodococcus)	+++	++
Yeast (S. Cerevisiae)	+++	++
Non-enveloped viruses (ADV)	+++	+++
Enveloped viruses (HSV-1)	+++	+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

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