DBdirect™ PCR SYBR Mix SuperSens [Discontinued]

(DB-1274)

This product has been replaced with new series of enhanced DBdirect™ PCR SYBR Mix SuperSens 2.0 and DBdirect™ PCR SYBR Mix SuperSens 2.0 UDG.


Introducing DBdirect™ PCR SYBR Mix SuperSens, a ready-to-use solution containing SYBR® Green I dye for real-time quantification of DNA sequences.


Perfect for routine and high throughput DNA target detection, this mix features our unique DBdirect™ technology, eliminating the need for DNA extraction and enhancing your workflow with greater accuracy and sensitivity through SuperSens technology.

Package size

Key Parameters: No need for NA extraction with unique specificity

Real Time PCR with antibody hot-start

Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order)

Product info

Cat. no.

Quantity

Lead Time

DBdirect™ PCR SYBR Mix SuperSens (DB-1274)

100 rxns

Discontinued

DBdirect™ PCR SYBR Mix SuperSens (DB-1274)

1000 rxns

Discontinued

DBdirect™ PCR SYBR Mix SuperSens (DB-1274)

5000 rxns

Discontinued

Details

Key Protein

Hot-start

Content

QC Assays

Taq DNA Polymerase

Antibody-based

DBdirect™ PCR SYBR Mix SuperSens (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

Downloads

Document name

Download

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms

DB-1274 with LBA

DB-1274 PCR Mix

Human Cells

+++

+++

Gram-negative bacteria (E. coli)

+++

+++

Gram-positive bacteria (Lactobacillus)

++

+++

Gram-positive bacteria (Rhodococcus)

+++

++

Yeast (S. Cerevisiae)

+++

+

Non-enveloped viruses (ADV)

+++

+++

Enveloped viruses (HSV-1)

+++

+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

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Let's take your PCR to the next level!

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