(DB-411201)
AT 590 is a bright orange fluorescent dye that is structurally identical to Atto™ 590. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.
Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AT 590 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.
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Key Parameters: Great brightness and pH stability with lower costs
High photostability: AT 590's rigid rhodamine structure prevents the formation of isomers in solution. Optical properties remain incredibly consistent regardless of temperature or solvent changes.
Applications: Confocal microscopy, super resolution microscopy (STED, PALM, dSTORM), flow cytometry (FACS), cell cultures and tissue labeling.
Lower cost alternative with protocol compatibility: Same Spectral Properties & Performance = Works exactly like Atto™ 590 with friendly price. Use it as a substitute for Alexa Fluor® 594 or Texas Red®.
Long and stable fluorescence lifetime: This makes it a great candidate for fluorescence lifetime imaging microscopy (FLIM) and time-gated detection systems, allowing you to easily filter out short-lived background autofluorescence from cells.
AT 590: Pick your modification
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Optical Data of AT 590
Name | Color | Excitation (nm) | Emission (nm) | Extinction coeficient (ε) | Cell Permeability |
|---|---|---|---|---|---|
AT 590 | Orange | 592 | 621 | 120 000 | Yes |
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