AT 590: Fluorescent Dye

(DB-411201)

AT 590 is a bright orange fluorescent dye that is structurally identical to Atto 590. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AT 590 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

Package size

Key Parameters: Great brightness and pH stability with lower costs

High photostability: AT 590's rigid rhodamine structure prevents the formation of isomers in solution. Optical properties remain incredibly consistent regardless of temperature or solvent changes.

Applications: Confocal microscopy, super resolution microscopy (STED, PALM, dSTORM), flow cytometry (FACS), cell cultures and tissue labeling.

Lower cost alternative with protocol compatibility: Same Spectral Properties & Performance = Works exactly like Atto™ 590 with friendly price. Use it as a substitute for Alexa Fluor® 594 or Texas Red®.

Long and stable fluorescence lifetime: This makes it a great candidate for fluorescence lifetime imaging microscopy (FLIM) and time-gated detection systems, allowing you to easily filter out short-lived background autofluorescence from cells.

AT 590: Pick your modification

Name (click for product information)

Availability

AT 590 NHS ester

In Stock

AT 590 Carboxylic Acid

In Stock

AT 590 Amine

In Stock

AT 590 Alkyne

In Stock

AT 590 Azide

In Stock

AT 590 PEG4 Alkyne

In Stock

AT 590 PEG4 Azide

In Stock

AT 590 DBCO

In Stock

AT 590 Maleimide

In Stock

AT 590 PEG5-Biotin

In Stock

AT 590 TCO

In Stock

Optical Data of AT 590

Name

Color

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

Cell Permeability

AT 590

Orange

592

621

120 000

Yes

AT 590: Absorption and emission spectra of AT 590

Absorption and emission spectra of AT 590:The absorption and emission spectra of AT 590 visualized. The absorption peak at 592 nm ensures efficient excitation, while the emission peak at 621 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AT 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

AT 590: Structure

AT 590:Structure presented as AT 590 NHS Ester

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