AF 488 NHS Ester: Fluorescent Dye

(DB-410102)

AF 488 is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.


The NHS Ester is an amine-reactive dye: It can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Package size

Key Parameters: Great brightness and pH stability with lower costs

NHS Ester: label amine groups in proteins, peptides or amino-modified oligos

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 488 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 488

Pricing

Cat. no.

Quantity

Price

Availability

DB-410102-1MG

1 mg

95 EUR

In stock

DB-410102-5MG

5 mg

245 EUR

In stock

DB-410102-10MG

10 mg

360 EUR

In stock

DB-410102-25MG

25 mg

715 EUR

In stock

DB-410102-50MG

50 mg

1220 EUR

In stock

DB-410102-100MG

100 mg

1580 EUR

In stock

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (95 %+ purity)

Optical Data of AF 488 NHS Ester

Name

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

MW (g/mol)

Formula

AF 488 NHS Ester

495

519

73 000

732.73

C31H32N4O13S2

AF 488 NHS Ester: Absorption and emission spectra

Absorption and emission spectra of AF 488:The absorption and emission spectra of AF 488 NHS Ester visualized. The absorption peak at 495 nm ensures efficient excitation, while the emission peak at 519 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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