AF 488 Azide: Fluorescent Dye

(DB-410106)

AF 488 Azide is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins.


AF 488 Azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.

Package size

Key Parameters: Great brightness and pH stability with lower costs

Azide: Reactive with terminal alkynes via a copper-catalyzed click reaction

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 488 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 488

Pricing

Cat. no.

Quantity

Price

Availability

DB-410106-1MG

1 mg

95 EUR

In stock

DB-410106-5MG

5 mg

245 EUR

In stock

DB-410106-10MG

10 mg

410 EUR

In stock

DB-410106-25MG

25 mg

715 EUR

In stock

DB-410106-50MG

50 mg

1220 EUR

In stock

DB-410106-100MG

100 mg

1570 EUR

In stock

General information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 488 Azide

Name

Excitation (nm)

Emission (nm)

Excitation coeficient (ε)

MW (g/mol)

Formula

AF 488 Azide

494

517

71 800

717.77

C30H35N7O10S2

AF 488 Azide: Absorption and emission spectra

Absorption and emission spectra of AF 488:The absorption and emission spectra of AF 488 Azide visualized. The absorption peak at 494 nm ensures efficient excitation, while the emission peak at 517 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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AF 488 Alkyne is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 Alkyne is used for fluorescent labeling of azidated biomolecules, polymers, and surfaces.

AF 488 PEG4-Azide is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Conjugates of molecules with AF 488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. The PEG4 linker works great for in-cell labeling.

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