AF 488 TCO: Fluorescent Dye

(DB-410112)

AF 488 TCO is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins.


AF 488 with trans-cyclooctene (TCO) readily reacts with tetrazines and possesses ultrafast kinetics, selectivity, and long-term aqueous stability, which is important in low-concentration applications such as protein-protein conjugations.

Package size

Key Parameters: Great brightness and pH stability with lower costs

TCO: Important for low concentration applications

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 488 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 488

General information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 488 Alkyne

Cat. no.

Quantity

Price

Availibility

Extinction coeficient (ε)

MW (g/mol)

Formula

DB-410112-1MG

1 mg

165 EUR

In stock

71 800

672.72

C30H32N4O10S2

DB-410112-5MG

5 mg

520 EUR

In stock

DB-410112-10MG

10 mg

790 EUR

In stock

DB-410112-25MG

50 mg

1440 EUR

In stock

DB-410112-50MG

100 mg

On request

In stock

DB-410112-100MG

On request

In stock

Optical Data of AF 488 TCO

Name

Excitation (nm)

Emission (nm)

Excitation coeficient (ε)

MW (g/mol)

Formula

AF-488 TCO

494

517

71 800

870.05

C42H55N5O11S2

AF 488 TCO: Absorption and emission spectra

Absorption and emission spectra of AF 488:The absorption and emission spectra of AF 488 TCO visualized. The absorption peak at 494 nm ensures efficient excitation, while the emission peak at 517 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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