AT 590 NHS Ester: Fluorescent Dye

(DB-411202)

AT 590 is a bright orange-fluorescent dye that is structurally identical to Atto 590. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AT 590 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.


The NHS Ester is an amine-reactive dye: It can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Fluorescence microscopy image of cells stained with AT 590

Package size

Fluorescence microscopy image of cells stained with AT 590

Key Parameters: Great brightness and pH stability with lower costs

NHS Ester: label amine groups in proteins, peptides or amino-modified oligos

Applications: Confocal microscopy, super resolution microscopy (STED, PALM, dSTORM), flow cytometry (FACS), cell cultures and tissue labeling

Lower cost alternative with protocol compatibility: Same Spectral Properties & Performance = Works exactly like Atto™ 590 with friendly price. Use it as a substitute for Alexa Fluor® 590 or Texas Red®.

Complete Compatibility – Can be used in any existing protocol that uses Atto 590

Pricing

Cat. no.

Quantity

Price

Lead Time

DB-411202-1MG

1 mg

150 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411202-5MG

5 mg

420 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411202-10MG

10 mg

630 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411202-25MG

25 mg

1200 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411202-50MG

50 mg

2025 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411202-100MG

100 mg

3000 EUR

1–2 days (CZ) / within 7 days (EU)

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (80 %+ purity)

Optical Data of AT 590 NHS Ester

Name

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Expected mass added after conjugation (g/mol)

AT 590 NHS Ester

592

621

120 000

572.7

AT 590 NHS Ester: Absorption and emission spectra

Spectrum of fluorescent dye AT 590
Absorption and emission spectra of AT 590:The absorption and emission spectra of AT 590 NHS Ester visualized. The absorption peak at 592 nm ensures efficient excitation, while the emission peak at 621 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AT 590 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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