Cyanine 5.5: Fluorescent Dye

(DB-411001)

Cyanine 5.5 is a near infrared (NIR) fluorescent dye that is structurally identical to Cy5.5. Known for its exceptionally high extinction coefficient and emission in the NIR spectrum, it is a premier choice for researchers needing to push beyond the limits of standard visible fluorophores.


Because NIR light significantly reduces scattering and absorption by biological tissues, Cyanine 5.5 offers extraordinary deep-tissue penetration. It is perfect for a variety of demanding applications, including in vivo small animal imaging, deep-tissue confocal microscopy, and highly multiplexed flow cytometry panels.

Chemical structure of Cyanine 5.5
Fluorescence microscopy image of cells stained with Cyanine 5.5

Package size

Chemical structure of Cyanine 5.5
Fluorescence microscopy image of cells stained with Cyanine 5.5

Key Parameters: Upgrade your in vivo assays with exceptional deep-tissue penetration and lower costs

Exceptional Brightness: Cyanine 5.5 features a massive molar extinction coefficient (typically >250,000 M⁻¹cm⁻¹). This highly efficient light absorption generates a brilliant near-infrared signal ideal for detecting low-abundance targets.

Applications: In vivo small animal imaging, deep-tissue confocal microscopy, flow cytometry (FACS), near-infrared Western blotting, and cell/tissue labeling.

Cost-effective protocol compatibility: Offers the exact same spectral properties and performance as standard Cy5.5 at a more accessible price. Serve as a direct, highly economical substitute for near-infrared dyes like Alexa Fluor® 680.

Superior Deep-Tissue Penetration: Emitting at 695 nm, Cyanine 5.5 operates in the "near-infrared window" where biological tissues are most highly transparent. This drastically reduces background autofluorescence and light scattering, allowing for crystal-clear imaging deep within intact tissues and live animals.

Cyanine 5.5: Pick your modification

Name (click for product information)

Price

Lead Time

Cyanine 5.5 NHS ester

From 95 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 Carboxylic Acid

From 95 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 Amine

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 Alkyne

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 Azide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 PEG4-Alkyne

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 PEG4-Azide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 DBCO

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 Maleimide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 PEG5-Biotin

From 130 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Cyanine 5.5 TCO

From 210 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Optical Data of Cyanine 5.5

Name

Color

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Cell Permeability

Cyanine 5.5

Near infrared (NIR)

678

695

250 000

Yes

Cyanine 5.5: Absorption and emission spectra of Cyanine 5.5

Excitation and emission spectra of Cyanine 5.5
Absorption and emission spectra of Cyanine 5.5:The absorption and emission spectra of Cyanine 5.5 visualized. The absorption peak at 678 nm ensures efficient excitation, while the emission peak at 695 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, Cyanine 5.5 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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