Cyanine 5.5: Fluorescent Dye

(DB-411001)

Cyanine 5.5 is a near infrared (NIR) fluorescent dye that is structurally identical to Cy5.5. Known for its exceptionally high extinction coefficient and emission in the NIR spectrum, it is a premier choice for researchers needing to push beyond the limits of standard visible fluorophores.


Because NIR light significantly reduces scattering and absorption by biological tissues, Cyanine 5.5 offers extraordinary deep-tissue penetration. It is perfect for a variety of demanding applications, including in vivo small animal imaging, deep-tissue confocal microscopy, and highly multiplexed flow cytometry panels.

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Key Parameters: Upgrade your in vivo assays with exceptional deep-tissue penetration and lower costs

Exceptional Brightness: Cyanine 5.5 features a massive molar extinction coefficient (typically >250,000 M⁻¹cm⁻¹). This highly efficient light absorption generates a brilliant near-infrared signal ideal for detecting low-abundance targets.

Applications: In vivo small animal imaging, deep-tissue confocal microscopy, flow cytometry (FACS), near-infrared Western blotting, and cell/tissue labeling.

Cost-effective protocol compatibility: Offers the exact same spectral properties and performance as standard Cy5.5 at a more accessible price. Serve as a direct, highly economical substitute for near-infrared dyes like Alexa Fluor® 680.

Superior Deep-Tissue Penetration: Emitting at 696 nm, Cyanine 5.5 operates in the "near-infrared window" where biological tissues are most highly transparent. This drastically reduces background autofluorescence and light scattering, allowing for crystal-clear imaging deep within intact tissues and live animals.

Cyanine 5.5: Pick your modification

Name (click for product information)

Availability

Cyanine 5.5 NHS ester

In Stock

Cyanine 5.5 Carboxylic Acid

In Stock

Cyanine 5.5 Amine

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Cyanine 5.5 Alkyne

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Cyanine 5.5 Azide

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Cyanine 5.5 PEG4 Alkyne

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Cyanine 5.5 PEG4 Azide

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Cyanine 5.5 DBCO

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Cyanine 5.5 Maleimide

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Cyanine 5.5 PEG5-Biotin

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Cyanine 5.5 TCO

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Optical Data of Cyanine 5.5

Name

Color

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

Cell Permeability

Cyanine 5.5

Far-Red

678

695

73 000

Yes

Cyanine 5.5: Absorption and emission spectra of Cyanine 5.5

Absorption and emission spectra of Cyanine 5.5:The absorption and emission spectra of Cyanine 5.5 visualized. The absorption peak at 678 nm ensures efficient excitation, while the emission peak at 695 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, Cyanine 5.5 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

Cyanine 5.5: Structure

Cyanine 5.5:Structure presented as Cyanine 5.5

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