AF 488: Fluorescent Dye

(DB-410100)

AF 488 is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

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Key Parameters: Great brightness and pH stability with lower costs

High photostability and pH stability

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 488 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 488

AF 488: Pick your modification

Name (click for product information)

Availability

AF 488 NHS ester

In Stock

AF 488 Carboxylic Acid

In Stock

AF 488 Amine

In Stock

AF 488 Alkyne

In Stock

AF 488 Azide

In Stock

AF 488 PEG4 Alkyne

In Stock

AF 488 PEG4 Azide

In Stock

AF 488 DBCO

In Stock

AF 488 Maleimid

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AF 488 PEG5-Biotin

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AF 488 TCO

In Stock

Optical Data of AF 488

Name

Color

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

Cell Permeability

AF 488

Green

495

519

73 000

Yes

AF 488: Absorption and emission spectra of AF 488

Absorption and emission spectra of AF 488:The absorption and emission spectra of AF 488 visualized. The absorption peak at 495 nm ensures efficient excitation, while the emission peak at 519 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 488 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

AF 488: Structure

AF 488:Structure presented as AF 488 NHS Ester

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Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.

AF 488-NHS Ester is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488-NHS Ester is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

AF 488 Alkyne is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 Alkyne is used for fluorescent labeling of azidated biomolecules, polymers, and surfaces.

AF 488 amine is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 Amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.

AF 488 Carboxylic Acid is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488 is perfect as a reference standard in experiments where AF 488 conjugates are used or can be used for synthesis of activated esters or variously modified.

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