AF 555: Fluorescent Dye

(DB-410501)

AF 555 is a bright yellow-fluorescent dye that is structurally identical to Alexa Fluor 555. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 555 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

Chemical structure of AF 555
Fluorescence microscopy image of neurons stained with AF 555

Package size

Chemical structure of AF 555
Fluorescence microscopy image of neurons stained with AF 555

Key Parameters: Great brightness and pH stability with lower costs

High photostability and pH stability

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 555 at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 555

AF 555: Pick your modification

Name (click for product information)

Price

Lead Time

AF 555 NHS ester

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 Carboxylic Acid

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 Amine

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 Alkyne

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 Azide

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 PEG4 Alkyne

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 PEG4 Azide

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 DBCO

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 Maleimide

From 115 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 PEG5-Biotin

From 145 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 555 TCO

From 232 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Optical Data of AF 555

Name

Color

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Cell Permeability

AF 555

Yellow

552

566

152 000

Yes

AF 555: Absorption and emission spectra of AF 555

Excitation and emission spectra of AF 555
Absorption and emission spectra of AF 555:The absorption and emission spectra of AF 555 visualized. The absorption peak at 552 nm ensures efficient excitation, while the emission peak at 566 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 555 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

AF 555 : Structure

Chemical structure of AF 555
AF 555:Structure of AF 555 fluorescent dye.

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