AF 350: Fluorescent Dye

(DB-411300)

AF 350 is a blue fluorescent dye that is structurally identical to Alexa Fluor 350. With its high photostability and pH resistance, it has become widely used fluorophores in life sciences.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 350 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

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Key Parameters: Great brightness and pH stability with lower costs

High photostability and pH stability

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 350 at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 350

AF 350: Pick your modification

Name (click for product information)

Availability

AF 350 NHS ester

1–2 days (CZ) / within 7 days (EU)

AF 350 Carboxylic Acid

1–2 days (CZ) / within 7 days (EU)

AF 350 Amine

1–2 days (CZ) / within 7 days (EU)

AF 350 Alkyne

1–2 days (CZ) / within 7 days (EU)

AF 350 Azide

1–2 days (CZ) / within 7 days (EU)

AF 350 PEG4 Alkyne

1–2 days (CZ) / within 7 days (EU)

AF 350 PEG4 Azide

1–2 days (CZ) / within 7 days (EU)

AF 350 DBCO

1–2 days (CZ) / within 7 days (EU)

AF 350 Maleimide

1–2 days (CZ) / within 7 days (EU)

AF 350 PEG5-Biotin

1–2 days (CZ) / within 7 days (EU)

AF 350 TCO

1–2 days (CZ) / within 7 days (EU)

Optical Data of AF 350

Name

Color

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Cell Permeability

AF 350

Blue

346

445

19 000

Depends on conjugate and reactive group

AF 350: Absorption and emission spectra of AF 350

Absorption and emission spectra of AF 350:The absorption and emission spectra of AF 350 visualized. The absorption peak at 346 nm ensures efficient excitation, while the emission peak at 445 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 350 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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