AF 350 NHS Ester: Fluorescent Dye

(DB-411302)

AF 350 is a blue fluorescent dye that is structurally identical to Alexa Fluor 350. With its high photostability and pH resistance, it is widely used in life science research.


Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 350 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.


The NHS Ester is an amine-reactive dye: It can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Package size

Key Parameters: Great brightness and pH stability with lower costs

NHS Ester: label amine groups in proteins, peptides or amino-modified oligos

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower cost alternative with protocol compatibility: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 350 at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 350

Pricing

Cat. no.

Quantity

Price

Availability

DB-411302-1MG

1 mg

155 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411302-5MG

5 mg

434 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411302-10MG

10 mg

651 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411302-25MG

25 mg

1240 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411302-50MG

50 mg

2093 EUR

1–2 days (CZ) / within 7 days (EU)

DB-411302-100MG

100 mg

3100 EUR

1–2 days (CZ) / within 7 days (EU)

Shipping information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (80 %+ purity)

Optical Data of AF 350 NHS Ester

Name

Excitation (nm)

Emission (nm)

Extinction coeficient (ε)

MW (g/mol)

Formula

AF 350 NHS Ester

346

445

19 000

TBD

TBD

AF 350 NHS Ester: Absorption and emission spectra

Absorption and emission spectra of AF 350:The absorption and emission spectra of AF 350 NHS Ester visualized. The absorption peak at 346 nm ensures efficient excitation, while the emission peak at 445 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 350 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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AF 488-NHS Ester is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 488-NHS Ester is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

AF 568-NHS Ester is a bright orange-fluorescent dye that is structurally identical to Alexa Fluor 568 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 568-NHS Ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.

AF 594 NHS Ester is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 NHS Ester. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 594 with NHS Esteris used for labeling proteins, peptides, antibodies, and any molecules containing an -NH2 group it results in the formation of stable amide bonds between the dye and the target molecule.

AF 647 NHS Ester is a bright far-red-fluorescent dye that is structurally identical to Alexa Fluor 647 NHS Ester. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 647 with NHS Ester is widely applied for imaging and flow cytometry.

AF 555-NHS Ester is a bright yellow-fluorescent dye that is structurally identical to Alexa Fluor 555 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 555-NHS Ester is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

AT 488 NHS Ester is a bright green-fluorescent dye that is structurally identical to Atto 488 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AT 488 NHS Ester is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

Cyanine 5 is a far-red fluorescent dye that is structurally identical to Cy5. With its high photostability and quantum yield for single-molecule applications, it has become one of the most widely used fluorophores in life sciences. Cyanine 5 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues. With the NHS Ester you can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

Cyanine 5.5 is a near infrared (NIR) fluorescent dye that is structurally identical to Cy5.5. Known for its exceptionally high extinction coefficient and emission in the NIR spectrum, it is a premier choice for researchers needing to push beyond the limits of standard visible fluorophores. With the NHS Ester you can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

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AT 590 NHS Ester is a bright orange-fluorescent dye that is structurally identical to Atto 590 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AT 590 NHS Ester is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

AF 700 NHS Ester is near-infrared fluorescent dye that is structurally identical to Alexa Fluor 700. Its excellent photostability and low background fluorescence make it well suited for multicolor flow cytometry and other fluorescence-based applications. AF 700 is excited by standard 633-640 nm red lasers and enables sensitive detection while preserving visible-spectrum channels for multiplex experiments.

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