(DB-410901)
Cyanine 5 is an intensely bright far-red fluorescent dye that is structurally identical to Cy5. Known for its exceptionally high extinction coefficient and strong signal generation, it remains one of the most fundamental and widely used fluorophores in life sciences.
Because it emits in the far-red spectrum, Cyanine 5 helps researchers completely avoid the natural background autofluorescence of cells and tissues. It is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), DNA microarrays, and highly multiplexed labeling panels.
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Key Parameters: Maximize your signal with no background
Exceptional Brightness: Cyanine 5 features a massive molar extinction coefficient (~250,000 M⁻¹cm⁻¹). This highly efficient light absorption generates an incredibly strong signal, making it ideal for detecting low-abundance targets.
Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), DNA/RNA microarrays, whole-animal imaging, cell cultures and tissue labeling.
Cost-effective protocol compatibility: Offers the exact same spectral properties and performance as standard Cy5 at a more accessible price. It serves as a direct, highly economical substitute for Alexa Fluor® 647 when extreme photostability is not strictly required.
Zero background for seamless multiplexing: Emitting in the far-red region (~662 nm), Cyanine 5 bypasses the natural green/yellow autofluorescence of biological samples. This guarantees a high signal-to-noise ratio and makes it the perfect anchor dye for multi-color multiplexing panels.
Cyanine 5: Pick your modification
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Optical Data of Cyanine 5
Name | Color | Excitation (nm) | Emission (nm) | Extinction coeficient (ε) | Cell Permeability |
|---|---|---|---|---|---|
Cyanine 5 | Red | 647 | 665 | 73 000 | Yes |
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