AF 594: Fluorescent Dye

(DB-410300)

AF 594 is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 which is commonly used for multiplexing. With stable signal generation and its pH insensitivity, its widely used in flow cytometry.

The red AF 594 is widely used in a range of SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy.

Package size

Key Parameters: Ideal for super resolution microscopy with lower costs

High fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity

Applications: Confocal Microscopy, SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy, Flow Cytometry (FACS)

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 594 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 594

AF 594 List of modifications

Name (click for product information)

Availability

AF 594 NHS ester

In Stock

AF 594 Carboxylic Acid

In Stock

AF 594 Amine

In Stock

AF 594 Alkyne

In Stock

AF 594 Azide

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AF 594 PEG4 Alkyne

In Stock

AF 594 PEG4 Azide

In Stock

AF 594 DBCO

In Stock

AF 594 Maleimid

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AF 594 PEG5 Biotin

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AF 594 TCO

In Stock

Optical Data of AF 594

Name

Color

Excitation (nm)

Emission (nm)

Excitation coeficient (ε)

Cell Permeability

AF 594

Red

590

618

105 000

Yes

AF 594: Absorption and emission spectra of AF 594

Absorption and emission spectra of AF 594:The absorption and emission spectra of AF 594 visualized. The absorption peak at 590 nm ensures efficient excitation, while the emission peak at 618 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 568 is ideal for flow cytometry, confocal microscopy and super resolution microscopy (STORM).

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Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.

AF 488 is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 488. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 488 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.

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