AF 594: Fluorescent Dye

(DB-410300)

AF 594 is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 which is commonly used for multiplexing. With stable signal generation and its pH insensitivity, it is widely used in flow cytometry.

The red AF 594 is widely used in a range of SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy.

Chemical structure of AF 594
Fluorescence microscopy image of cells stained with AF 594

Package size

Chemical structure of AF 594
Fluorescence microscopy image of cells stained with AF 594

Key Parameters: Ideal for super resolution microscopy with lower costs

High fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity

Applications: Confocal Microscopy, SRM applications including dSTORM, structured illumination microscopy (SIM), stimulated emission depletion (STED) microscopy and in two-photon excitation (TPE) microscopy, Flow Cytometry (FACS)

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 594 at a more accessible price

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 594

AF 594: Pick your modification

Name (click for product information)

Price

Lead Time

AF 594 NHS ester

From 95 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 Carboxylic Acid

From 95 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 Amine

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 Alkyne

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 Azide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 PEG4 Alkyne

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 PEG4 Azide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 DBCO

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 Maleimide

From 105 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 PEG5 Biotin

From 130 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

AF 594 TCO

From 210 EUR / 1 mg

1–2 days (CZ) / within 7 days (EU)

Optical Data of AF 594

Name

Color

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

Cell Permeability

AF 594

Red

590

618

105 000

Yes

AF 594: Absorption and emission spectra of AF 594

Excitation and emission spectra of AF 594
Absorption and emission spectra of AF 594:The absorption and emission spectra of AF 594 visualized. The absorption peak at 590 nm ensures efficient excitation, while the emission peak at 618 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 594 is ideal for flow cytometry, confocal microscopy and super resolution microscopy (STORM).

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