AF 594 PEG4-Alkyne: Fluorescent Dye

(DB-410307)

AF 594 PEG4-Alkyne is a bright orange-fluorescent dye that is structurally identical to Alexa Fluor 594. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins.


PEG4 spacer improves accessibility to binding partners in click chemistry and reduces nonspecific interactions with hydrophobic surfaces/proteins. It makes it better for in-cell labeling, protein conjugation, or any application where spatial flexibility, solubility, or reduced nonspecific binding is critical.

Fluorescence microscopy image of cells stained with AF 594

Package size

Fluorescence microscopy image of cells stained with AF 594

Key Parameters: High fluorescence quantum yield with lower costs

PEG4-Alkyne: Reactive with azides via a copper-catalyzed click reaction

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 594 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 594

Pricing

Cat. no.

Quantity

Price

Lead Time

DB-410307-1MG

1 mg

105 EUR



1–2 days (CZ) / within 7 days (EU)

DB-410307-5MG

5 mg

294 EUR



1–2 days (CZ) / within 7 days (EU)

DB-410307-10MG

10 mg

441 EUR



1–2 days (CZ) / within 7 days (EU)

DB-410307-25MG

25 mg

840 EUR




1–2 days (CZ) / within 7 days (EU)

DB-410307-50MG

50 mg

1418 EUR




1–2 days (CZ) / within 7 days (EU)

DB-410307-100MG

100 mg

2100 EUR



1–2 days (CZ) / within 7 days (EU)

General Information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 594 PEG4-Alkyne

Name

Excitation (nm)

Emission (nm)

Extinction coefficient (ε)

MW (g/mol)

Formula

AF 594 PEG4-Alkyne

590

618

105 000

1138.44

C58H83N5O14S2

AF 594 PEG4-Alkyne: Absorption and emission spectra

Excitation and emission spectra of AF 594
Absorption and emission spectra of AF 594:The absorption and emission spectra of AF 594 PEG4-Alkyne visualized. The absorption peak at 590 nm ensures efficient excitation, while the emission peak at 618 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 594 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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