(DB-410303)
AF 594 Carboxylic Acid is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins.
The carboxylic acid group can also be utilized for the synthesis of activated esters—such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol), and STP (4-sulfo-2,3,5,6-tetrafluorophenol)—or modified with hydrazines, hydroxylamines, or amines in aqueous media using water-soluble carbodiimides. This allows conjugation to molecules containing amino groups, including proteins, antibodies, and peptides. Consequently, AF 488 carboxylic acid is commonly used during solid-phase peptide synthesis to modify peptides in situ in the presence of activating agents like HATU., a red-fluorescent dye, reacts with azides via a copper-catalyzed click reaction.
Package size
Key Parameters: Stable signal generation and pH stability with lower costs
Carboxylic group: Ideal as a reference standard or for further synthesis
Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling
Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 594 with friendly price.
Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 594
Pricing
Cat. no. | Quantity | Price |
|---|---|---|
DB-410303-1MG | 1 mg | 95 EUR |
DB-410303-5MG | 5 mg | 245 EUR |
DB-410305-10MG | 10 mg | 410 EUR |
DB-410303-25MG | 25 mg | 710 EUR |
DB-410303-50MG | 50 mg | 1220 EUR |
DB-410303-100MG | 100 mg | 1565 EUR |
General Information
Shipping | Storage | Quality Control |
|---|---|---|
Room Temperature | Up to 24 months in -20 ℃ | HPLC (98 %+ purity) |
Optical Data of AF 594 Carboxylic Acid
Name | Appearance | Excitation (nm) | Emission (nm) | Excitation coeficient (ε) | MW (g/mol) | Formula |
|---|---|---|---|---|---|---|
AF 594 Carboxylic Acid | Black-blue crystals | 590 | 618 | 105 000 | 925.16 | C47H64N4O11S2 |
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AF 594
AF 594 is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 which is commonly used for multiplexing. With stable signal generation and its pH insensitivity, its widely used in flow cytometry and super resolution microscopy such as STORM.
AF 594 Alkyne
AF 594 alkyne is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 568 Alkyne can be reacted with azides via a copper-catalyzed click reaction.
AF 594 Amine
AF 594 Amine is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 594 Amine is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues
