AF 594 Alkyne: Fluorescent Dye

(DB-410305)

AF 594 Alkyne is a bright orange-fluorescent dye that is structurally identical to Alexa Fluor 594. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins.


AF 594 Alkyne, a red-fluorescent dye, reacts with azides via a copper-catalyzed click reaction. Click chemistry uses bio-orthogonal tags—unique to biological systems—for highly specific, efficient labeling. This two-step reaction forms a stable triazole without side reactions, extreme conditions, or interference from complex biological environments. It is an ideal solution for wide range of imaging techniques.

Package size

Key Parameters: High fluorescence quantum yield with lower costs

Alkyne: Reactive with azides via a copper-catalyzed click reaction

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 594 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 594

Pricing

Cat. no.

Quantity

Price

DB-410305-1MG

1 mg

95 EUR



DB-410305-5MG

5 mg

245 EUR



DB-410305-10MG

10 mg

410 EUR



DB-410305-25MG

25 mg

710 EUR




DB-410305-50MG

50 mg

1220 EUR




DB-410305-100MG

100 mg

1565 EUR



General Information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 594 Alkyne

Name

Appearance

Excitation (nm)

Emission (nm)

Excitation coeficient (ε)

MW (g/mol)

Formula

AF 594 Alkyne

Red Solid

590

618

105 000

962.23


C50H67N5O10S2

AF 594 Alkyne: Absorption and emission spectra

Absorption and emission spectra of AF 594:The absorption and emission spectra of AF 594 Alkyne visualized. The absorption peak at 590 nm ensures efficient excitation, while the emission peak at 618 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 594 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.

AF 594 is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 594 which is commonly used for multiplexing. With stable signal generation and its pH insensitivity, its widely used in flow cytometry and super resolution microscopy such as STORM.

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