Next-Gen DB RT-PCR Mixes 2.0: Unmatched accuracy and sensitivity

DB RT-PCR Mixes 2.0 are the ultimate solution for efficient and reproducible analysis. Our mixes are designed to meet your high standards for quality and performance with exceptional accuracy provided by our SuperSens technology. All the 2.0 mixes are based on our proprietary enzymes, DBscript Reverse Transcriptase and DB AptaTaq DNA Polymerase supported by our own DB RNase Inhibitors.


Probe or SYBR? Choose the best for your purposes! All the mixes are offered with the DBdirect™ technology which will help you reduce the total handling time and costs.


Our RT-PCR Mixes are available also as Lyo-Ready product with the same final composition!


We created the complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors. Find everything you need in one place. Download it HERE.

DBdirect™ RT-PCR SYBR Mix (DB-1265) kit, 1000 reactions
DBdirect™ RT-PCR SYBR Mix (DB-1265) kit, 1000 reactions

PRODUCTS

DB RT-PCR Mixes 2.0: Meet our unique solutions DBdirect™ and SuperSens!

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 (DB-1321) kit box and tubes

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 (DB-1323) kit box and tubes

New generation of DBdirect™ RT-PCR SYBR 2.0 mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG (DB-1322) kit box and tubes

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1324) kit box and tubes

New generation of DBdirect™ RT-PCR SYBR 2.0 UDG mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

DB RT-PCR Probe Mix SuperSens 2.0 (DB-1312) kit box and tubes

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR SYBR Mix SuperSens 2.0 (DB-1315) kit box and tubes

Next-generation of DB RT-PCR SYBR Mix SuperSens is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR Probe Mix SuperSens 2.0 UDG (DB-1313) kit box and tubes

Next-generation of DB RT-PCR Probe Mix SuperSens UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1318) kit box and tubes

New-generation of DB RT-PCR SYBR Mix SuperSens UDG is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR Probe Mix SuperSens 2.0 dUTP (DB-1314) kit box and tubes

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 (DB-1321) kit box and tubes

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology! Now available in lyo-ready version and enhanced formulation with dedicated primer stabilizing solution for improve primer stability at ambient temperature.

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 (DB-1321) kit box and tubes

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology! Now available in lyo-ready version and flexible reconstitution using either 2X or 4X buffer, allowing users to select the preferred workflow setup.

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG (DB-1322) kit box and tubes

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology! Now available in lyo-ready version and enhanced formulation with dedicated primer stabilizing solution for improve primer stability at ambient temperature.

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG (DB-1322) kit box and tubes

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology! Now available in lyo-ready version and flexible reconstitution using either 2X or 4X buffer, allowing users to select the preferred workflow setup.

DB Lysis Buffer A (DB-1281) bottle

DB Lysis Buffer B is suitable for a wide range of samples. Extract your RNA easily from different cell types.

Key Parameters

Explore our DB RT-PCR Mixes 2.0 with unique technologies: DBdirect™ and SuperSens

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DBdirect™ Technology


DBdirect™: Skip RNA extraction with direct RT-PCR detection.

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Various Applications


Versatile Applications: Suitable for quantitative real-time RT-PCR from RNA, cDNA, DNA, and various sample types, including human cells, bacteria, viruses, and biological matrices like serum and saliva.

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Supersens Technology


Achieve exceptional sensitivity with our SuperSens technology!

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Wide Range of Samples


Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

"Our mixes are designed to meet your high standards for quality and performance, making it an essential tool for your laboratory. You can prepare a multiplex or singleplex reaction from the portfolio above and take your RT-PCR to the next level!"

Eva Mlynarčíková

Lead Developer in PCR chemistry

Selection Guide

DB RT-PCR Mixes 2.0

Catalogue Number

TaqMan® Compatible

SYBR® Green I

Ultrasensitive Detection

RT-PCR of Long Amplicons

dUTP and UDG Included

Direct Detection

Single-Cell Gene Expression Analysis

DB RT-PCR Probe Mix SuperSens 2.0

DB-1312

✅

❌

✅

✅

❌

❌

❌

DB RT-PCR Probe Mix SuperSens 2.0 UDG

DB-1313

✅

❌

✅

✅

✅

❌

❌

DB RT-PCR SYBR Mix SuperSens 2.0

DB-1315

❌

✅

✅

✅

❌

❌

❌

DB RT-PCR SYBR Mix SuperSens 2.0 UDG

DB-1318

❌

✅

✅

✅

✅

❌

❌

DBdirect™ RT-PCR Probe Mix SuperSens 2.0

DB-1312

✅

❌

✅

✅

❌

✅

✅

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG

DB-1322

✅

❌

✅

✅

✅

✅

✅

DBdirect RT-PCR SYBR Mix SuperSens 2.0

DB-1323

❌

✅

✅

✅

❌

✅

✅

DBdirect™ RT-PCR SYBR Mix SuperSens UDG

DB-1324

❌

✅

✅

✅

✅

✅

✅

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