We are devoted to improve direct RT-PCR and other RNA-based applications and we understand know important it is to protect RNA from degradation.
RNase inhibitors are the only way to protect RNA across a wide range of applications. However, all protein inhibitors are highly unstable, while synthetic inhibitors have low potency and low assay compatibility. As a result, none of them reliably protect RNA.
That is why we developed DB Ultima H RNase inhibitor – a breakthrough, highly engineered human RNase inhibitor with optimal inhibition profile and outstanding stability. It is the most potent inhibitor of human RNases and potently inhibits also other eucaryotic and E.coli RNases. It sets a new benchmark in reliability through unprecedented oxidative resistance and stability across a wide range of assay conditions. It strongly outperforms other inhibitors in direct RT-PCR, RNA-seq, in vitro transcription and other applications.
Our portfolio also features a unique DB Bovine RNase inhibitor. All our inhibitors are of exceptional purity and recombinantly expressed in E. coli.
We created the complete catalogue of our RNase inhibitors, PCR and RT-PCR mixes and enzymes. Find everything you need HERE.
PRODUCTS
DB Ultima H RNase Inhibitor
Unique approach to the RNase inhibition! Bovine variant offers a remarkable stability with fewer cysteine residues, ensuring consistent performance and oxidation resistance.
RNase Inhibitor Bovine
Unique approach to the RNase inhibition! Bovine variant offers a remarkable stability with fewer cysteine residues, ensuring consistent performance and oxidation resistance.
RNase Inhibitor Human
The best option for samples of human origin against RNases A, B and C. Recombinant protein from E. coli
RNase Inhibitor Porcine
The best option for your everyday work. The golden standard of RNase inhibition.
Key Parameters
Retains activity without DTT. Fully active even at 2% H₂O₂.
The only protein RNase inhibitor stable above 55 ℃!
Stable at room temperature in usual PCR buffers for months.
Even higher activity than human RNase inhibitor in human tissues!
Selection Guide
Name (Cat. no.) | Inhibition of Human RNases | Oxidative stability | Storage stability | Thermal stability | Origin |
|---|---|---|---|---|---|
Recombinant | |||||
DB RNase Inhibitor Human (DB-1259) | Recombinant | ||||
DB RNase Inhibitor Bovine (DB-1260) | Recombinant | ||||
DB RNase Inhibitor Porcine (DB-1261) | Recombinant |
References
Exploring Advanced Methodologies for NS5 Methyltransferase Targeted Drug Discovery, Evžen Bouřa Group, IOCB Prague