DB Ultima H RNase Inhibitor: The ultimate RNase inhibitor

DB Ultima H RNase Inhibitor

(DB-1306)

Scientists often blame the RNA for bad results. But in most cases the RNA is just a victim. Your RNA didn't fail. Your RNase inhibitor did!

Protect your RNA against degradation with our DB Ultima H RNase Inhibitor, the ultimate solution for RNase inhibition!, offering exceptional resistance to oxidation.

Highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market!

See the complete info in our DB Ultima H RNase Inhibitor leaflet!

Package size

Key Parameters: The most potent and stable inhibitor on the market

Thermal Stability: The only protein inhibitor stable at or above 55°C.

High Potency: Highest possible inhibition of human RNases. Inhibits also other eukaryotic and E.coli RNases.

Extraordinary Resilience: Stable for months at room temperature. Fully active without DTT, cannot be oxidized. Compatible with the widest range of buffers & pH. Survives multiple freeze/thaw cycles.

Direct RT-PCR ready: Even higher activity than human RNase inhibitor in human tissues. Higher temperature ensure increased yields and sensitivity.

DB Ultima H RNase Inhibitor has superior oxidative stability: The only truly oxidatively resistant protein inhibitor on the market

DB Ultima H is the only oxidatively resistant inhibitor:Bovine and porcine inhibitors provide some resistance. Human and rat inhibitors are very limited in resistance and murine inhibitors have no resistance. This leaves the DB Ultima H the only truly oxidative resistant inhibitor even with 2% peroxide use. Numbers represent the same order of RNase inhibitors as it is in the first graph.

Truly reliable inhibitor: The only RNase inhibitor active across variou conditions

Freeze/thaw cycle stability:Left: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1mM DTT after a single freeze/thaw cycle at -20℃. Right: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1 mM DTT after 24 hours on wet ice.

DB Ultima H RNase Inhibitor thermostability: The only inhibitor on the market active above 55°C

Residual RNase Inhibitor activity after 5 min incubation at 55 ℃ in optimal buffer :DB Ultima H will enable RT-PCR in direct RT-qPCR at higher temperature; increasing yields and sensitivity 55°C is the optimal temperature for mutated M-MLV reverse transcriptase and optimal for UDG system.

Pricing

Cat. No.	Quantity	Price	Lead Time
DB Ultima H RNase Inhibitor (DB-1306)	2.5 kU	100 EUR	In Stock
DB Ultima H RNase Inhibitor (DB-1306)	10 kU	308 EUR	In Stock
DB Ultima H RNase Inhibitor (DB-1306)	40 kU	985 EUR	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate	0.5 mM EDTA 20 mM HEPES 7.5, 50 mM KCl, 8 mM DTT, 50% glycerol	SDS-PAGE/purity Functional Assay (PCR) DNase, RNase, Endonuclease activity absence DNA content absence E. coli gDNA absence

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate

0.5 mM EDTA

20 mM HEPES 7.5,

50 mM KCl, 8 mM DTT, 50% glycerol

SDS-PAGE/purity

Functional Assay (PCR)

DNase, RNase, Endonuclease activity absence

DNA content absence

E. coli gDNA absence

Downloads

Document name	Download

Order or request free sample

Customers who explored this product also showed interest in:

RNase Inhibitor Human

The best option for samples of human origin against RNases A, B and C. Recombinant protein from E. coli

RNase Inhibitor Porcine

The best option for your everyday work. The golden standard of RNase inhibition.

RNase Inhibitor Bovine

Unique approach to the RNase inhibition! Bovine variant offers a remarkable stability with fewer cysteine residues, ensuring consistent performance and oxidation resistance.

Let's take your PCR to the next level!

Write us

[email protected]