DB Ultima H RNase Inhibitor: Absolute RNA Protection

(DB-1306)

Scientists often blame the RNA for bad results. But in most cases the RNA is just a victim. Your RNA didn't fail. Your RNase inhibitor did!


Protect your RNA against degradation with our DB Ultima H RNase Inhibitor, the ultimate solution for RNase inhibition, offering exceptional resistance to oxidation.


Highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market!


See the complete info in our DB Ultima H RNase Inhibitor leaflet!

Package size

Key Parameters: How does DB Ultima H improve your RNA protection?

Thermal Stability: The only protein inhibitor stable at or above 55°C.

High Potency: Highest possible inhibition of human RNases. Inhibits also other eukaryotic and E.coli RNases.

Extraordinary Resilience: Stable for months at room temperature. Fully active without DTT, cannot be oxidized. Compatible with the widest range of buffers & pH. Survives multiple freeze/thaw cycles.

Direct RT-PCR ready: Even higher activity than human RNase inhibitor in human tissues. Higher temperature ensure increased yields and sensitivity.

Stop relying on unstable DTT to protect your RNA. DB Ultima H is the only inhibitor fully active without it.

DB Ultima H is the only oxidatively resistant inhibitor:Standard inhibitors on the market are very limited in oxidative resistance. This leaves the DB Ultima H the only truly oxidative resistant inhibitor even with 2% hydrogen peroxide use with no DTT. Numbers represent the same order of RNase inhibitors as it is in the first graph.

DB Ultima H stays fully active through freeze/thaw cycles that completely destroy standard inhibitors.

Freeze/thaw cycle stability:Left: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1mM DTT after a single freeze/thaw cycle at -20℃. Right: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1 mM DTT after 24 hours on wet ice.

Maximize your RT-PCR sensitivity and yields: DB Ultima H stays fully active at 55°C, a temperature that completely destroys standard inhibitors.

Residual RNase Inhibitor activity after 5 min incubation at 55 ℃ in optimal buffer :DB Ultima H will enable RT-PCR in direct RT-qPCR at higher temperature; increasing yields and sensitivity 55°C is the optimal temperature for mutated M-MLV reverse transcriptase and optimal for UDG system.

Applications: DB Ultima H ensures RNA protection with no enzyme interference

Single-Cell Profiling & In Vitro Assays: Full RNA integrity guaranteed for prolonged in vitro protocols and single-cell expression profiling with zero enzyme interference.

RNA Structural & Functional Studies: Work across broad pH ranges and without DTT to establish perfect native conditions, completely free from the risk of sample degradation.

Direct RT-PCR from Human Samples: Bypass purification and amplify directly from crude lysates. Exceptional potency and oxidative resistance neutralize aggressive, RNase-rich environments in complex human tissues.

cDNA Synthesis & Cloning: Guarantee full-length transcripts. Safely melt complex secondary RNA structures at 55°C to maximize cDNA synthesis yields, without compromising downstream enzymatic steps.

Pricing

Cat. No.

Quantity

Price

Lead Time

DB Ultima H RNase Inhibitor (DB-1306)

2.5 kU

100 EUR

In Stock

DB Ultima H RNase Inhibitor (DB-1306)

10 kU

308 EUR

In Stock

DB Ultima H RNase Inhibitor (DB-1306)

40 kU

985 EUR

In Stock

Details

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate

0.5 mM EDTA

20 mM HEPES 7.5,

50 mM KCl, 8 mM DTT, 50% glycerol

SDS-PAGE/purity

Functional Assay (PCR)

DNase, RNase, Endonuclease activity absence

DNA content absence

E. coli gDNA absence

Downloads

Document name

Download

Order or request free sample

I agree to the Processing of Personal Data

I agree to the Terms and Conditions

Related Products

Customers who explored this product also showed interest in:

Unique approach to the RNase inhibition! Bovine variant offers a remarkable stability with fewer cysteine residues, ensuring consistent performance and oxidation resistance.

The DBscript 2.0 cDNA Synthesis Kit is an all-inclusive, optimized solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-sensitive, ~10-fold faster mutant enzyme, use this kit to confidently detect low copy targets and accelerate your prompt analysis!

The DBscript Turbo cDNA Synthesis Kit is an optimized, all-inclusive solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-fast, highly processive mutant enzyme, use this kit to power through long transcripts and accelerate your workflows with speeds up to 100-fold faster than legacy standards!

DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Next-generation of DB RT-PCR SYBR Mix SuperSens is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New-generation of DB RT-PCR SYBR Mix SuperSens UDG is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New generation of DBdirect™ RT-PCR SYBR 2.0 UDG mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

New generation of DBdirect™ RT-PCR SYBR 2.0 mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

Next-generation of DB RT-PCR Probe Mix SuperSens UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Let's take your PCR to the next level!

Write us

[email protected]