DB Ultima H RNase Inhibitor: The ultimate RNase inhibitor

DB Ultima H RNase Inhibitor

(DB-1306)

Scientists often blame the RNA for bad results. But in most cases the RNA is just a victim. Your RNA didn't fail. Your inhibitor did!

Protect your RNA against degradation with our DB Ultima H RNase Inhibitor, the ultimate solution for RNase inhibition!, offering exceptional resistance to oxidation.

Highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market!

See the complete info in our DB Ultima H RNase Inhibitor leaflet!

Package size

Key Parameters: The only potent and stable inhibitor on the market

Fully oxidative resistant: Retains acitivity without DTT. Fully active even at 2% H2O2.

Thermal stability: The only protein RNase inhibitor stable above 55°C.

Storage stability: Stable for more than months at room temperature. Compatible with different PCR buffers: no risk of transmission of infectious agents.

Direct RT-PCR ready: Even higher activity than human RNase inhibitor in human tissues. Higher temperature ensure increased yields and sensitivity.

DB Ultima H RNase Inhibitor stability: The most stable inhibitor in comparison by DSF

Melting temperatures of RNase Inhibitors in PCR buffer determined by differential scanning fluorimetry (DSF):Melting temperature (Tm) is the temperature, where approximately 50% of the protein is denatured, rapid denaturation above the Tm follows. DB Ultima H has approximately 8 to 10°C higher Tm than murine or porcine inhibitors and 3 to 6°C higher Tm than human or bovine inhibitors. All inhibitors except DB Ultima H have Tm below 55°C (including any other engineered version from any organism).

DB Ultima H RNase Inhibitor oxidative stability: The only truly oxidatively resistant protein inhibitor on the market

Residual activity after pre-incubation with hydrogen peroxide (H2O2) for 20 min:Bovine and porcine inhibitors provide some resistance. Human and rat inhibitors are very limited in resistance and Murine inhibitors have no resistance. This leaves the DB Ultima H the only truly oxidative resistant inhibitor even with 2% H202 use. Numbers represent the same order of RNase inhibitors as it is in the first graph.

DB Ultima H RNase Inhibitor thermostability: The only inhibitor on the market active above 55°C

Residual RNase Inhibitor activity after 5 min incubation at 55 ℃ in optimal buffer :DB Ultima H will enable RT-PCR in direct RT-qPCR at higher temperature; increasing yields and sensitivity 55°C is the optimal temperature for mutated M-MLV reverse transcriptase and optimal for UDG system.

Pricing

Cat. No.	Quantity	Price	Lead Time
DB Ultima H RNase Inhibitor (DB-1306)	2.5 kU	100 EUR	In Stock
DB Ultima H RNase Inhibitor (DB-1306)	10 kU	308 EUR	In Stock
DB Ultima H RNase Inhibitor (DB-1306)	40 kU	985 EUR	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate	0.5 mM EDTA 20 mM HEPES 7.5, 50 mM KCl, 8 mM DTT, 50% glycerol	SDS-PAGE/purity Functional Assay (PCR) DNase, RNase, Endonuclease activity absence DNA content absence E. coli gDNA absence

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate

0.5 mM EDTA

20 mM HEPES 7.5,

50 mM KCl, 8 mM DTT, 50% glycerol

SDS-PAGE/purity

Functional Assay (PCR)

DNase, RNase, Endonuclease activity absence

DNA content absence

E. coli gDNA absence

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Let's take your PCR to the next level!

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