(DB-1306)
RNase inhibitors are the only way to protect RNA in native samples. However, all protein inhibitors are highly unstable, while synthetic inhibitors have low potency and low assay compatibility. As a result, none of them reliably protects RNA.
That is why we developed DB Ultima H RNase inhibitor – a breakthrough, highly engineered human RNase inhibitor with the highest potency and unprecedented stability. It is fully oxidatively resistant and does not need reducing agents and as a result, it is stable and active across all relevant assay and storage conditions. Moreover, it is thermostable and can be kept at room temperature. It is the most potent inhibitor of human RNases and is the best option for protecting RNA in human samples.
It potently inhibits also other eukaryotic RNases and is effective against E.coli RNases. It outperforms other RNase inhibitors in RT-PCR, direct RT-PCR, cDNA synthesis, RNA-seq library preparation, in vitro transcription and other applications!
The DB Ultima H RNase Inhibitor is available also as Lyo-Ready product! To receive more info, please contact us through a form below.
See the complete info in our DB Ultima H RNase Inhibitor leaflet!
Package size
Key Parameters: How does DB Ultima H improve your RNA protection?
Extraordinary stability: Room temperature stable. Survives multiple freeze/thaw cycles and is stable in long-term storage. The most stable RNase inhibitor available.
Thermal Stability: The only protein inhibitor stable at or above 55°C. Compatible with reverse transcription protocols at up to 65°C.
High Potency: Potently inhibits eukaryotic RNases. Inhibits also and E.coli RNases.
Direct RT-PCR ready: Exceptionally potent inhibition of human RNases.
Broadest compatibility: Does not inhibit DNA polymerases or reverse transcriptases. Active across wide range of buffers & pH. Active without DTT.
Applications: DB Ultima H ensures RNA protection with no enzyme interference
cDNA Synthesis & Cloning: Efficient protection of RNA leading to increased yield of full-length transcripts. Compatible with reverse transcriptase step up to 65°C to maximize yield from structured RNAs. Additionaly, DB Ultima H increases the efficacy of the transcriptase.
Direct RT-PCR from Human Samples: Bypass purification and amplification directly from crude lysates. Exceptional potency and oxidative resistance ensure reliable RNA protection even in difficult samples.
Single-Cell RT-PCR and RNA-seq Profiling: highly efficient RNA protection and compatibility with direct one-step RT-PCR protocols from the cells without the need for DTT and without interfering with other enzymes in the workflow.
RNA Structural & Functional Studies: Work across broad pH ranges and without DTT to reliable inhibit RNases and prevent sample degradation.
Pricing: Order with Us
Cat. No. | Quantity | Price | Lead Time |
|---|---|---|---|
DB Ultima H RNase Inhibitor (DB-1306) | 2.5 kU | 100 EUR | 1-2 days (CZ) / within 7 days (EU) |
DB Ultima H RNase Inhibitor (DB-1306) | 10 kU | 308 EUR | 1-2 days (CZ) / within 7 days (EU) |
DB Ultima H RNase Inhibitor (DB-1306) | 40 kU | 985 EUR | 1-2 days (CZ) / within 7 days (EU) |
Find Your Distributor
Czechia, Slovakia and Hungary | UK and Ireland | Italy | Germany, Switzerland and Austria | Northern Europe | Benelux, Spain and Portugal |
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Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit inhibits 5 ng of RNase A by 50% using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate | 0.5 mM EDTA 20 mM HEPES 7.5, 50 mM KCl, 8 mM DTT, 50% glycerol | SDS-PAGE/purity Functional Assay (PCR) DNase, RNase, Endonuclease activity absence DNA content absence E. coli gDNA absence |
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