(DB-1277)
Ensure excellent results in your PCR and RT-PCR applications with DB AptaTaq DNA Polymerase. Perfect for routine PCR amplification, high throughput PCR, TA cloning, and DNA labeling, this thermostable enzyme (half-life over 40 minutes at 95 °C) features an aptamer-based hot-start for easy preparation at ambient temperature. It provides 5’→3′ polymerase and exonuclease activities, essential for hydrolysis probes like TaqMan™, and generates PCR products with 3′-dA overhangs for TA cloning.
Package size
Key Parameters: Achieve Superior PCR and RT-PCR Results with DB AptaTaq DNA Polymerase
Thermostable Taq DNA Polymerase: Derived from Thermus aquaticus with a half-life of over 40 minutes at 95 °C.
Aptamer-Based Hot-Start: Simplifies preparation of PCR mix at ambient temperature. Provides long-term sample storage
Enzymatic Activities: Includes 5’→3′ polymerase activity, 5’→3′ exonuclease activity (essential for use with hydrolysis probes like TaqMan™), and 5′ flap activity. No detectable 3’→5′ exonuclease activity.E. coli: no risk of transmission of infectious agents
TA Cloning: Generates PCR products with 3′ -dA overhangs.
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
|---|---|---|---|
DB AptaTaq DNA Polymerase (DB-1277) | 0.1 kU | 20 EUR | In Stock |
DB AptaTaq DNA Polymerase (DB-1277) | 0.5 kU | 80 EUR | In Stock |
DB AptaTaq DNA Polymerase (DB-1277) | 2.5 kU | 330 EUR | In Stock |
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes the incorporation of 10 nmol of dNTPs into 250 nM fluorophore-quencher dual-labelled extendable DNA molecular beacon within 30 min at 72 °C. | 20 mM Tris 8, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% Igepal CA-630, 0.5% Tween, 50% glycerol | SDS-PAGE/purity Functional Assay (PCR) DNase, RNase, Endonuclease activity absence DNA content absence E. coli gDNA absence |
Downloads
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