DBdirect™ RT-PCR SYBR Mix SuperSens: Greater accuracy

DBdirect RT-PCR SYBR Mix SuperSens

(DB-1269)

Introducing DBdirect™ RT-PCR SYBR Mix, the perfect solution for routine and high-throughput RNA target detection. Featuring SYBR® Green I dye for real-time quantification, this mix combines the advantages of direct RT-PCR with no RNA extraction needed, enhanced by our SuperSens technology for greater accuracy and sensitivity.

Trusted in millions of COVID-19 diagnostic tests. Experience fast and efficient workflows with the ready-to-use DBdirect™ and SuperSens Technology mix, eliminating the need for TaqMan probes!

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Key Parameters: Revolutionize RNA Detection with DBdirect™ RT-PCR SYBR Mix

Ready to use mix for fast and comfortable workflows with the DBdirect™ and SuperSens Technology!

SYBR® Green I dye included in the mix: No TaqMan probes are needed

Hot-start: Antibody. Various applications including qualitative and quantitative real-time RT-PCR from RNA

Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™ RT-PCR SYBR Mix SuperSens (DB-1269)	100 rxns	120 EUR	In Stock
DBdirect™ RT-PCR SYBR Mix SuperSens (DB-1269)	1000 rxns	640 EUR	In Stock
DBdirect™ RT-PCR SYBR Mix SuperSens (DB-1269)	5000 rxns	2360 EUR	In Stock

Details

Key Proteins	Hot-start	Content	QC Assays
Taq DNA Polymerase Thermostable reverse transcriptase (mutated M-MLV) RNase Inhibitors	Antibody-based	Enhancer mix SuperSens (4x) DBdirect™ RT-PCR SYBR Mix SuperSens (4x) PCR Grade Water	Functional test (RT-PCR) E. coli gDNA absence RNase and DNase absence Activity measurement of key proteins

Key Proteins

Hot-start

Content

QC Assays

Taq DNA Polymerase

Thermostable reverse transcriptase (mutated M-MLV)

RNase Inhibitors

Antibody-based

Enhancer mix SuperSens (4x)

DBdirect™ RT-PCR SYBR Mix SuperSens (4x)

PCR Grade Water

Functional test (RT-PCR)

E. coli gDNA absence

RNase and DNase absence

Activity measurement of key proteins

Sensitive single cell detection with DBdirect RT-PCR SYBR Mix SuperSens

Profile the transcripts of interest to determine cell differentiation, stemness, marker expression, mutations, and more with no need for pre-amplification and RNA isolation:The DBdirect™ technology in our RT-PCR mixes allows for direct lysis and qRT-PCR in a single well. The streamlined protocol requires no RNA isolation or pre-amplification. In this study we measured transcripts in FACS sorted single cells directly into the PCR mixes. The results demonstrate that DBdirect™ RT-PCR mixes are sensitive enough to allow transcriptional profiling of single cells. Human cells (CCRF-CEM) were FACS sorted directly into 384-well plates containing 10 µl of the RT-PCR mixes, and subjected to 1-step qRT-PCR. Two assays consisting of primers and probes for GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) and TBP (TATA-box-binding protein) were used for the RT-PCR probe mix. GAPDH is a high-abundance transcript with thousands copies per cell, TBP is a low-abundance transcript with tens of copies per cell based on the protein atlas cell line expression data (if we assume 10 million transcripts per cell). Histograms of Ct values for GAPDH and TBP (n = 60 wells per PCR mix) revealed approximately log-normal distributions, indicating reliable detection. Grey bars indicate wells with no Ct values (also attributable to FACS errors). Results show approximately log-normal distributions of the Ct values, suggesting a reliable detection.

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