DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG

DBdirect^™ RT-PCR SYBR Mix SuperSens UDG

(DB-1324)

Introducing new generation DBdirect™ RT-PCR SYBR Mix 2.0 UDG with adjusted robustness and sensitivity. The perfect solution for routine and high-throughput RNA target detection. Featuring SYBR® Green I dye for real-time quantification, this mix combines the advantages of direct RT-PCR with no RNA extraction needed.

Trusted in millions of COVID-19 diagnostic tests. Experience fast and efficient workflows with the ready-to-use DBdirect™ and SuperSens Technology mix, eliminating the need for TaqMan probes!

The 2.0 series of RT-PCR Mixes is exceptionally sensitive and robust. Our mixes can detect work with 1 DNA copy. Its stability and user friendly packaging is ideal for everyday analysis. All mixes in the 2.0 series are suitable for single-cell detection. Contains UDG (uracil-DNA glycosylase to prevent carryover contamination from previous PCR reactions.

Package size

Key Parameters: Revolutionize RNA Detection with DBdirect™ RT-PCR SYBR Mix 2.0 UDG

Exceptionally robust and sensitive: The 2.0 series of DB RT-PCR Mixes can detect approx. 1 copy of DNA.

Reverse Transcriptase warm-start complements proprietary, ultra-efficient antibody double hot-start of Taq Polymerase

Various applications including qualitative and quantitative real-time RT-PCR from RNA

Stable for at least days at 25°C, at least several weeks at 4°C

Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).

UDG variant contains optimal ratio of dTTP and dUTP to maximize robustness without compromising UDG efficiency.

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1324)	100 rxns	135 EUR	In Stock
DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1324)	1000 rxns	1172 EUR	In Stock
DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1324)	5000 rxns (5x1000 rxns)	4980 EUR	In Stock
DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1324)	10 000 rxns	Upon request	Upon request

Details

Key Proteins	Hot-start	Content	QC Assays
DB AptaTaq DNA Polymerase DBscript Reverse Transcriptase (M-MLV) RNase Inhibitor Bovine	Reverse transcriptase warm-start; antibody-based hot-start of Taq Polymerase.	DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (4x) PCR Grade Water	Functional test (RT-PCR) E. coli gDNA absence RNase and DNase absence Activity measurement of key proteins

Key Proteins

Hot-start

Content

QC Assays

DB AptaTaq DNA Polymerase

DBscript Reverse Transcriptase (M-MLV)

RNase Inhibitor Bovine

Reverse transcriptase warm-start; antibody-based hot-start of Taq Polymerase.

DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG (4x)

PCR Grade Water

Functional test (RT-PCR)

E. coli gDNA absence

RNase and DNase absence

Activity measurement of key proteins

Sensitive single cell detection with DBdirect RT-PCR SYBR Mix SuperSens

Profile the transcripts of interest to determine cell differentiation, stemness, marker expression, mutations, and more with no need for pre-amplification and RNA isolation:The DBdirect™ technology in our RT-PCR mixes allows for direct lysis and qRT-PCR in a single well. The streamlined protocol requires no RNA isolation or pre-amplification. In this study we measured transcripts in FACS sorted single cells directly into the PCR mixes. The results demonstrate that DBdirect™ RT-PCR mixes are sensitive enough to allow transcriptional profiling of single cells. Human cells (CCRF-CEM) were FACS sorted directly into 384-well plates containing 10 µl of the RT-PCR mixes, and subjected to 1-step qRT-PCR. Two assays consisting of primers and probes for GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) and TBP (TATA-box-binding protein) were used for the RT-PCR probe mix. GAPDH is a high-abundance transcript with thousands copies per cell, TBP is a low-abundance transcript with tens of copies per cell based on the protein atlas cell line expression data (if we assume 10 million transcripts per cell). Histograms of Ct values for GAPDH and TBP (n = 60 wells per PCR mix) revealed approximately log-normal distributions, indicating reliable detection. Grey bars indicate wells with no Ct values (also attributable to FACS errors). Results show approximately log-normal distributions of the Ct values, suggesting a reliable detection.

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