(DB-1318)
Introducing new generation of DB RT-PCR SYBR Mix SuperSens 2.0 UDG, specifically designed for SYBR Green I Dye® for real-time quantification.
Our mix streamlines your process and supports various applications including qualitative and quantitative real-time RT-PCR from RNA. Trusted in millions of COVID-19 diagnostic tests.
The 2.0 series of RT-PCR Mixes is exceptionally sensitive and robust. Our mixes can detect work with 1 DNA copy. Its stability and user friendly packaging is ideal for everyday analysis. All mixes in the 2.0 series are suitable for single-cell detection. Contains UDG (uracil-DNA glycosylase to prevent carryover contamination from previous PCR reactions.
Package size
Key Parameters: Achieve Superior Accuracy with DB RT-PCR SYBR Mix SuperSens 2.0 UDG
Exceptionally robust and sensitive: The 2.0 series of DB RT-PCR Mixes can detect approx. 1 copy of DNA.
Reverse Transcriptase warm-start complements antibody & aptamer double hot-start of Taq Polymerase
Various applications including qualitative and quantitative real-time RT-PCR from RNA
Stable for at least days at 25°C, at least several weeks at 4°C
Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).
UDG variant contains optimal ratio of dTTP and dUTP to maximize robustness without compromising UDG efficiency.Reverse Transcriptase with optimum at 50 to 55 °C for higher yields and full compatibility with thermolabile UDG enzyme
Pricing
Cat. no. | Quantity | Price | Lead Time |
|---|---|---|---|
DB RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1318) | 100 rxns | 114 EUR | In Stock |
DB RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1318) | 1000 rxns | 994 EUR | In Stock |
DB RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1318) | 5000 rxns (5x1000 rxns) | 4226 EUR | In Stock |
DB RT-PCR SYBR Mix SuperSens 2.0 UDG (DB-1318) | 10 000 rxns | Upon request | Upon request |
Details
Key Proteins | Hot-start | Content | QC Assays |
|---|---|---|---|
Reverse transcriptase warm-start; antibody & aptamer double hot-start of Taq Polymerase. | DB RT-PCR SYBR Mix SuperSens UDG (4x) PCR Grade Water | Functional test (RT-PCR) E. coli gDNA absence RNase and DNase absence Activity measurement of key proteins |
Testimonials
"The DB RT-PCR SYBR Mix SuperSens kit is ideal for direct and immediate use with freshly lysed cells, making RT-qPCR workflows more efficient. It delivers consistent qualitative analysis of RNA transcription in our CRISPR-Cas9 modified cell studies, allowing us to rapidly verify knockout success and proceed with work."
Downloads
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