DIANA Biotechnologies | DBScript Turbo cDNA Synthesis Kit

DBscript Turbo cDNA Synthesis Kit: Maximum Speed and Processivity for Complete cDNA Synthesis

(DB-1335)

Achieve unprecedented results with the DBscript Turbo cDNA Synthesis Kit. This advanced, complete kit pairs our unique, highly oxidation-resistant DB Ultima H RNase inhibitor with the DBscript Turbo Reverse Transcriptase, an ultra-fast M-MLV mutant engineered for superior processivity and the rapid synthesis of long, full-length cDNA libraries.

Enjoy industry-leading performance with optimum reverse transcription at 50–60 °C, and extreme thermostability that supports RT steps at up to an impressive 70 °C to resolve the most challenging RNA secondary structures. Benefit from a streamlined workflow that operates ~100-fold faster than every other cDNA synthesis kit on the market, while delivering enhanced sensitivity for your complex and low copy number targets.

Download the PDF catalogue filled with case studies here!

Package size

Key Parameters: Complete cDNA synthesis with ultra-sensitive reverse transcriptase

Maximum Sensitivity: Best-in-class performance for low copy number targets thanks to the DBscript 2.0 Reverse Transcriptase, ensuring reliable detection down to ~1 copy.

Exceptional Speed: Operates ~10-fold faster than SuperScript™ III or III Flash, drastically reducing your synthesis protocol time.

Enhanced Thermostability: Optimum activity between 50–60 °C, with robust functionality for RT steps at up to 65 °C.

Complete RNA Protection: Includes a unique DB Ultima H RNase inhibitor with exceptional oxidation resistance to completely safeguard sensitive RNA templates.

General info

Name (Cat. no.)	Quantity	Lead Time
DBscript 2.0 cDNA Synthesis Kit (DB-1334)	10 rxns	In Stock
DBscript 2.0 cDNA Synthesis Kit (DB-1334)	50 rxns	In Stock

Details

Content	Storage Buffer	QC Assays
DBscript 2.0 cDNA Synthesis Mix Mix (2X) Oligo(dT)20 primer (50 μM), random hexamers (125 μM) and a PCR grade water	20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol	Functional Assay (cDNA synthesis)

Content

Storage Buffer

QC Assays

DBscript 2.0 cDNA Synthesis Mix Mix (2X)

Oligo(dT)20 primer (50 μM), random hexamers (125 μM) and a PCR grade water

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

Functional Assay (cDNA synthesis)

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

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DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.

DB Oligo(dT)20 Primer

Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.

DB Anchored Oligo(dT)20-VN Primer

Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.

DBscript 2.0 Reverse Transcriptase

DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.

DBscript Turbo Reverse Transcriptase

DBscript Turbo is an ultra-fast, thermostable M-MLV mutant engineered for maximum processivity and efficiency. Operating ~100-fold faster than legacy enzymes and active up to 70°C, it is the premier choice for long transcripts, full-length cDNA library synthesis, and ultra-short diagnostic protocols.

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