Uracil DNA Glycosylases (UDG) are widely used in PCR and RT-qPCR workflows to prevent carry-over contamination originating from previous amplification reactions. By selectively removing uracil from DNA molecules, UDG enzymes ensure that contaminating PCR products cannot serve as templates in subsequent reactions, helping reduce false-positive results and improve assay reliability.
We offer a portfolio of thermolabile UDG enzymes optimized for modern molecular biology and diagnostic applications. Whether your workflow requires robust contamination control in routine PCR assays, compatibility with RT-qPCR protocols, or support for lyophilized reagent development, our UDG products provide reliable performance and convenient formulation options.
The portfolio includes standard glycerol formulations as well as glycerol-free Lyo-Ready variants for assay developers and manufacturers seeking freeze-dried solutions.
We created the complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors. Find everything you need in one place. Download it HERE.
PRODUCTS
DB Thermolabile UDG for PCR 100X
DB Thermolabile UDG for PCR 100X provides effective protection against PCR carry-over contamination in qPCR applications. It combines robust activity, exceptional stability in reaction mixes, and complete inactivation during amplification, enabling reliable results without interference with reverse transcription.
DB Thermolabile UDG for PCR Lyo-Ready 100X
DB Thermolabile UDG for PCR Lyo-Ready 100X provides effective protection against PCR carry-over contamination in qPCR applications. It combines robust activity, exceptional stability in reaction mixes, and complete inactivation during amplification, enabling reliable results without interference with reverse transcription. Lyo-Ready formulation offers seamless integration into lyophilized qPCR and diagnostic reagent mixes.
DB Thermolabile UDG for RT-PCR 100X
DB Thermolabile UDG for RT-PCR 100X provides effective protection against RT-PCR carry-over contamination in RT-qPCR applications. Optimized for modern one-step RT-qPCR workflows, it combines robust activity, exceptional stability in reaction mixes, and complete inactivation during amplification, enabling reliable results without interference with reverse transcription.
DB Thermolabile UDG for RT-PCR Lyo-Ready 100X
DB Thermolabile UDG for RT-PCR 100X provides effective protection against RT-PCR carry-over contamination in RT-qPCR applications. Optimized for modern one-step RT-qPCR workflows, it combines robust activity, exceptional stability in reaction mixes, and complete inactivation during amplification, enabling reliable results without interference with reverse transcription. Lyo-Ready formulation offers seamless integration into lyophilized qPCR and diagnostic reagent mixes.
Key Parameters
Selectively removes uracil-containing DNA from previous amplification reactions, helping eliminate false-positive results and improve confidence in PCR data.
Portfolio includes products optimized for both standard PCR workflows and RT-qPCR assays with reverse transcription requirements.
Selected UDG enzymes are available in glycerol-free Lyo-Ready formats to support freeze-dried assay development and manufacturing.
Choose from multiple UDG variants and formulations to match research, diagnostic, and OEM assay development needs.
DIANA Biotechnologies | OEM Partnership
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