(DB-1332)
Upgrade your reverse transcription workflow with DBscript 2.0 Reverse Transcriptase, an advanced, ultra-sensitive M-MLV mutant. Engineered to outperform standard enzymes, DBscript 2.0 delivers ~10-fold faster reaction times and higher thermostability than every competitor on the market.
Whether you are performing routine syntheses or detecting low copy number targets down to ~1 copy, this robust enzyme ensures reliable, maximum-yield cDNA synthesis for all your critical 1-step RT-PCR and diagnostic applications.
Package size
Key Parameters: The best solution for most applications
Maximum Sensitivity: Specifically optimized for low copy number targets, successfully detecting down to ~1 copy.
Exceptional Speed: Performs ~10-fold faster than Superscript III or III Flash.
High Thermostability: Features an optimum activity range of 50-60°C and maintains functionality for RT steps at up to 65°C.
Uncompromised Yield: Consistently delivers the same or better cDNA yield when compared to Superscripts.
General info
Name (Cat. no.) | Quantity | Lead Time |
|---|---|---|
DBscript 2.0 Reverse Transcriptase (DB-1332) | 2 kU | In Stock |
DBscript 2.0 Reverse Transcriptase (DB-1332) | 10 kU | In Stock |
DBscript 2.0 Reverse Transcriptase (DB-1332) | 40 kU | In Stock |
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C. | 20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol | SDS-PAGE/purity Functional Assay (cDNA synthesis) |
Downloads
Document name | Download |
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