Our DBscript portfolio offers high-performance solutions for your RNA workflows, available as standalone enzymes or comprehensive synthesis kits.
To match your highest expectations, we have created new generation of RTs with enhanced sensitivity or unprecedented speed. Are you looking for a robust enzyme for a routine applications or an ultrasensitive variant for advanced experiments? Choose your prefered variant:
DBscript 2.0: Engineered as an ultra-sensitive, thermostable mutant that operates up to ~10-fold faster than legacy market standards. It is optimized for low copy number targets (down to ~1 copy) and features a warm-start option, making it ideal for 1-step RT-PCR and diagnostics.
DBscript Turbo: Designed for extreme processivity and maximum speed, this ultra-fast mutant synthesizes cDNA ~100-fold faster than traditional enzymes and maintains exceptional stability for RT steps up to 70°C. It is the premier choice for long transcripts and rapid, full-length cDNA library synthesis.
We created the complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors. Find everything you need in one place. Download it HERE.
PRODUCTS
DBscript 2.0 Reverse Transcriptase
DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.
DBscript Turbo Reverse Transcriptase
DBscript Turbo is an ultra-fast, thermostable M-MLV mutant engineered for maximum processivity and efficiency. Operating ~100-fold faster than legacy enzymes and active up to 70°C, it is the premier choice for long transcripts, full-length cDNA library synthesis, and ultra-short diagnostic protocols.
DBscript Reverse Transcriptase
DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.
DBscript 2.0 cDNA Synthesis Kit
The DBscript 2.0 cDNA Synthesis Kit is an all-inclusive, optimized solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-sensitive, ~10-fold faster mutant enzyme, use this kit to confidently detect low copy targets and accelerate your prompt analysis!
DBscript Turbo cDNA Synthesis Kit
The DBscript Turbo cDNA Synthesis Kit is an optimized, all-inclusive solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-fast, highly processive mutant enzyme, use this kit to power through long transcripts and accelerate your workflows with speeds up to 100-fold faster than legacy standards!
DBscript cDNA Synthesis Kit
DBscript cDNA Synthesis Kit is an optimized kit containing all components needed for efficient synthesis of first-strand complementary DNA (cDNA) from mRNA or total RNA templates. Use our extremely fast protocol for your prompt analysis!
DB Anchored Oligo(dT)20-VN Primer
Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.
DB Oligo(dT)20 Primer
Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.
DB Random Hexamers
Make your cDNA synthesis kit with our proprietary DB Random Hexamers.
Key Parameters
Our enzymes provide superior stability, efficiency, and simplicity ensuring reliable results for every experiment.
Features an engineered MMLV mutant to synthesize long cDNAs (>9 kb). Confidently analyze a massive dynamic range of RNA inputs, from 1 μg down to just 1 pg.
Streamline your workflows with reduced pipetting steps and a highly optimized, ultra-fast 10-minute protocol perfectly suited for two-step RT-PCR.
Successfully power through GC-rich regions and complex RNA secondary structures thanks to enhanced protein thermostability and an exclusive buffer formulation.E. coli
Recombinantly expressed in E. coli (zero risk of infectious agents). The complete kit includes optimized buffers, dNTPs, DTT, and our unique, highly oxidation-resistant bovine RNase inhibitor.
Eva Mlynarčíková
Lead Developer in PCR chemistry
