Our DBscript portfolio offers high-performance solutions for your RNA workflows, available as a standalone enzymes or a comprehensive synthesis kit.
Powered by an advanced MMLV mutant with reduced RNase H activity, DBscript Reverse Transcriptase ensures exceptional cDNA synthesis, delivering higher yields and the ability to transcribe long RNA fragments (> 9 kb). The enzyme boasts remarkable thermostability, supporting reverse transcription at elevated temperatures (50–60 °C) to easily resolve complex RNA secondary structures.
For maximum convenience and accuracy, the DBscript cDNA Synthesis Kit streamlines your workflow. It features an ultra-fast 10-minute protocol for two-step RT-PCR, optimized buffers, and our unique bovine RNase inhibitor with exceptional oxidation resistance to completely protect your sensitive RNA templates.complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors.
We created the complete catalogue of our PCR and RT-PCR mixes, enzymes and inhibitors. Find everything you need in one place. Download it HERE.
PRODUCTS
Reverse Transcriptase
DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.
cDNA Synthesis Kit
DBscript cDNA Synthesis Kit is an optimized kit containing all components needed for efficient synthesis of first-strand complementary DNA (cDNA) from mRNA or total RNA templates. Use our extremely fast protocol for your prompt analysis!
DB Anchored Oligo(dT)20-VN Primer
Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.
DB Oligo(dT)20 Primer
Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.
DB Random Hexamers
Make your cDNA synthesis kit with our proprietary DB Random Hexamers.
Key Parameters
Our enzymes provide superior stability, efficiency, and simplicity ensuring reliable results for every experiment.
Features an engineered MMLV mutant to synthesize long cDNAs (>9 kb). Confidently analyze a massive dynamic range of RNA inputs, from 1 μg down to just 1 pg.
Streamline your workflows with reduced pipetting steps and a highly optimized, ultra-fast 10-minute protocol perfectly suited for two-step RT-PCR.
Successfully power through GC-rich regions and complex RNA secondary structures thanks to enhanced protein thermostability and an exclusive buffer formulation.E. coli
Recombinantly expressed in E. coli (zero risk of infectious agents). The complete kit includes optimized buffers, dNTPs, DTT, and our unique, highly oxidation-resistant bovine RNase inhibitor.
Eva Mlynarčíková
Lead Developer in PCR chemistry
