DBscript 2.0 cDNA Synthesis Kit: Unmatched Sensitivity for Complete cDNA Synthesis

(DB-1334)

Achieve superior results with the DBscript 2.0 cDNA Synthesis Kit. This advanced, complete kit features our unique, highly oxidation-resistant DB Ultima H RNase inhibitor paired with the next-generation DBscript 2.0 Reverse Transcriptase, an ultra-sensitive M-MLV mutant engineered for maximum diagnostic performance.


Enjoy exceptional reliability with reverse transcription at an optimum 50–60 °C, and sustained performance up to 65 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast protocol that operates ~10-fold faster than other enzymes on the market, providing the ultimate, streamlined solution for detecting low copy number targets (down to ~1 copy) in your critical assays.reverse transcription at 50-60 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast 10-minute protocol for two-step RT-PCR and optimized buffers for seamless one-step RT-PCR.

Package size

Key Parameters: Complete cDNA synthesis with ultra-sensitive reverse transcriptase

Maximum Sensitivity: Best-in-class performance for low copy number targets thanks to the DBscript 2.0 Reverse Transcriptase, ensuring reliable detection down to ~1 copy.

Exceptional Speed: Operates ~10-fold faster than SuperScript™ III or III Flash, drastically reducing your synthesis protocol time.

Enhanced Thermostability: Optimum activity between 50–60 °C, with robust functionality for RT steps at up to 65 °C.

Complete RNA Protection: Includes a unique DB Ultima H RNase inhibitor with exceptional oxidation resistance to completely safeguard sensitive RNA templates.

General info

Name (Cat. no.)

Quantity

Lead Time

DBscript 2.0 cDNA Synthesis Kit (DB-1334)

10 rxns

In Stock

DBscript 2.0 cDNA Synthesis Kit (DB-1334)

50 rxns

In Stock

Details

Content

Storage Buffer

QC Assays

DBscript 2.0 cDNA Synthesis Mix Mix (2X)


Oligo(dT)20 primer (50 μM), random hexamers (125 μM) and a PCR grade water

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

Functional Assay (cDNA synthesis)

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

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DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.

Make your cDNA synthesis kit with our proprietary DB Oligo(dT)20 Primer.

Make your cDNA synthesis kit with our proprietary DB Anchored Oligo(dT)20-VN Primer.

DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.

DBscript Turbo is an ultra-fast, thermostable M-MLV mutant engineered for maximum processivity and efficiency. Operating ~100-fold faster than legacy enzymes and active up to 70°C, it is the premier choice for long transcripts, full-length cDNA library synthesis, and ultra-short diagnostic protocols.

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