DBscript 2.0 cDNA Synthesis Kit: Unmatched Sensitivity for Complete cDNA Synthesis

(DB-1334)

Achieve superior results with the DBscript 2.0 cDNA Synthesis Kit. This advanced, complete kit features our unique, highly oxidation-resistant DB Ultima H RNase inhibitor paired with the next-generation DBscript 2.0 Reverse Transcriptase, an ultra-sensitive M-MLV mutant engineered for maximum diagnostic performance.


Enjoy exceptional reliability with reverse transcription at an optimum 50–60 °C, and sustained performance up to 65 °C, made possible by enhanced protein thermostability and our exclusive buffer formulation. Benefit from an ultra-fast protocol that operates ~10-fold faster than other enzymes on the market, providing the ultimate, streamlined solution for detecting low copy number targets (down to ~1 copy) in your critical assays.


Download the PDF catalogue filled with case studies here!

Package size

Key Parameters: Complete cDNA synthesis with ultra-sensitive reverse transcriptase

Maximum Sensitivity: Best-in-class performance for low copy number targets thanks to the DBscript 2.0 Reverse Transcriptase, ensuring reliable detection down to ~1 copy.

Exceptional Speed: Operates ~10-fold faster than SuperScript™ III or III Flash, drastically reducing your synthesis protocol time.

Enhanced Thermostability: Optimum activity between 50–60 °C, with robust functionality for RT steps at up to 65 °C.

Complete RNA Protection: Includes a unique DB Ultima H RNase inhibitor with exceptional oxidation resistance to completely safeguard sensitive RNA templates.

General info

Name (Cat. no.)

Quantity

Lead Time

DBscript 2.0 cDNA Synthesis Kit (DB-1334)

10 rxns

In Stock

DBscript 2.0 cDNA Synthesis Kit (DB-1334)

50 rxns

In Stock

Details

Content

Storage Buffer

QC Assays

DBscript 2.0 cDNA Synthesis Mix Mix (2X)


Oligo(dT)20 primer (50 μM), random hexamers (125 μM) and a PCR grade water

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

Functional Assay (cDNA synthesis)

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

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Temperature-Dependent cDNA Yield Comparison:DBscript 2.0 and DBscript Turbo consistently produced the highest cDNA yields from bacterial ribosomal RNA across all tested temperatures. While all reverse transcriptases showed limited activity after 1 minute, DBscript 2.0 and particularly DBscript Turbo already generated measurable amounts of cDNA, demonstrating superior performance under rapid reaction conditions. After 10 minutes, both enzymes achieved near-complete cDNA synthesis at elevated temperatures (60–65°C), substantially outperforming conventional reverse transcriptases from Thermo, Roche, NEB, and Takara. The ability of DBscript enzymes to maintain high activity at increased temperatures supports more efficient transcription of structured RNA templates, resulting in higher cDNA yields and improved reaction specificity.

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DBscript Reverse Transcriptase is essential for the synthesis of the complementary DNA (cDNA) strand from the RNA template. Use our Reverse Transcriptase in both two-step and one-step RT-PCR to ensure excellent results in your applications.

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DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.

DBscript Turbo is an ultra-fast, thermostable M-MLV mutant engineered for maximum processivity and efficiency. Operating ~100-fold faster than legacy enzymes and active up to 70°C, it is the premier choice for long transcripts, full-length cDNA library synthesis, and ultra-short diagnostic protocols.

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