DB Ultima H RNase Inhibitor Lyo-Ready

DB Ultima H RNase Inhibitor: Absolute RNA Protection in Lyo-Ready Variant

(DB-1307)

RNase inhibitors are the only way to protect RNA in native samples. However, all protein inhibitors are highly unstable, while synthetic inhibitors have low potency and low assay compatibility. As a result, none of them reliably protects RNA.

That is why we developed DB Ultima H RNase inhibitor – a breakthrough, highly engineered human RNase inhibitor with the highest potency and unprecedented stability. It is fully oxidatively resistant and does not need reducing agents and as a result, it is stable and active across all relevant assay and storage conditions. Moreover, it is thermostable and can be kept at room temperature. It is the most potent inhibitor of human RNases and is the best option for protecting RNA in human samples.

It potently inhibits also other eukaryotic RNases and is effective against E.coli RNases. It outperforms other RNase inhibitors in RT-PCR, direct RT-PCR, cDNA synthesis, RNA-seq library preparation, in vitro transcription and other applications!

DB Ultima H RNase Inhibitor is now available in a Lyo-Ready formulation for seamless integration into lyophilized RT-PCR and diagnostic reagent mixes.

See the complete info in our DB Ultima H RNase Inhibitor leaflet!

Package size

Key Parameters: How does DB Ultima H improve your RNA protection?

Extraordinary stability: Room temperature stable. Survives multiple freeze/thaw cycles and is stable in long-term storage. The most stable RNase inhibitor available.

Thermal Stability: The only protein inhibitor stable at or above 55°C. Compatible with reverse transcription protocols at up to 65°C.

High Potency: Potently inhibits eukaryotic RNases. Also inhibits other E. coli RNases.

Direct RT-PCR ready: Exceptionally potent inhibition of human RNases.

Broadest compatibility: Does not inhibit DNA polymerases or reverse transcriptases. Active across wide range of buffers & pH. Active without DTT.

Lyo-Ready: Glycerol-free and detergent-free RNase inhibitor formulation designed for incorporation into lyophilized molecular biology and diagnostic reagent mixes.

Ambient-temperature shipping: Reduces cold-chain packaging and transport emissions, supporting more sustainable delivery.

We have solved the fundamental issue of protein inhibitors: DB Ultima H is oxidatively resistant!

DB Ultima H is the only oxidatively resistant inhibitor:In our comparative study, we tested 14 major RNase inhibitors. Using our RNase inhibition assay, we determined the residual activity of each inhibitor (fraction of active inhibitor) after incubation with various levels of oxidative agent (hydrogen peroxide). As illustrated in the chart, all 14 inhibitors showed rapid decline of activity and virtually zero residual activity in 2% hydrogen peroxide, whereas DB Ultima H showed ~100% of its activity in these conditions.

Oxidative resistance gives DB Ultima H outstanding stability across relevant conditions

Freeze/thaw cycle stability:Up: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1mM DTT after a single freeze/thaw cycle at -20℃. Down: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1 mM DTT after 24 hours on wet ice.

DB Ultima H offers improved thermostability beating all other protein RNase inhibitors

DB Ultima H is the only inhibitor stable at 55°C:All other tested inhibitors showed > 90% decline in their activity after 5 min at 55°C, whereas DB Ultima H retained all of its activity. Additionally, we have determined that DB Ultima H retains most of its activity for at least 1 hour at 55°C (data not shown).

Applications: DB Ultima H ensures RNA protection with no enzyme interference

cDNA Synthesis & Cloning: Efficient protection of RNA leading to increased yield of full-length transcripts. Compatible with reverse transcriptase step up to 65°C to maximize yield from structured RNAs. Additionally, DB Ultima H increases the efficacy of the transcriptase.

Direct RT-PCR from Human Samples: Bypass purification and amplification directly from crude lysates. Exceptional potency and oxidative resistance ensure reliable RNA protection even in difficult samples.

Single-Cell RT-PCR and RNA-seq Profiling: highly efficient RNA protection and compatibility with direct one-step RT-PCR protocols from the cells without the need for DTT and without interfering with other enzymes in the workflow.

RNA Structural & Functional Studies: Work across broad pH ranges and without DTT to reliably inhibit RNases and prevent sample degradation.

Pricing

Cat. No.	Quantity	Lead Time
DB Ultima H RNase Inhibitor Lyo-Ready (DB-1307)	10 kU	1–2 days (CZ) / within 7 days (EU)
DB Ultima H RNase Inhibitor Lyo-Ready (DB-1307)	40 kU	1–2 days (CZ) / within 7 days (EU)

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount required to inhibit 5 ng of RNase A activity by 50 %, using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate. Activity is measured in 100 mM Tris acetate pH 6.5, 0.5 mM EDTA buffer, at 25 °C	0.5 mM EDTA 20 mM HEPES pH = 7.5, 50 mM KCl, 8 mM DTT	Activity of the RNase inhibitor. Purity ≥ 90%, determined by SDS-PAGE. Absence of contaminating nucleases, including DNase, RNase, latent RNase, and endonuclease activity. Absence of E. coli genomic DNA.

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount required to inhibit 5 ng of RNase A activity by 50 %, using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate. Activity is measured in 100 mM Tris acetate pH 6.5, 0.5 mM EDTA buffer, at 25 °C

0.5 mM EDTA

20 mM HEPES pH = 7.5,

50 mM KCl, 8 mM DTT

Activity of the RNase inhibitor.

Purity ≥ 90%, determined by SDS-PAGE.

Absence of contaminating nucleases, including DNase, RNase, latent RNase, and endonuclease activity.

Absence of E. coli genomic DNA.

Downloads

Document name	Download

Order or request free sample

Customers who explored this product also showed interest in:

RNase Inhibitor Bovine

Unique approach to the RNase inhibition! Bovine variant offers a remarkable stability with fewer cysteine residues, ensuring consistent performance and oxidation resistance.

DBscript 2.0 cDNA Synthesis Kit

The DBscript 2.0 cDNA Synthesis Kit is an all-inclusive, optimized solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-sensitive, ~10-fold faster mutant enzyme, use this kit to confidently detect low copy targets and accelerate your prompt analysis!

DBscript Turbo cDNA Synthesis Kit

The DBscript Turbo cDNA Synthesis Kit is an optimized, all-inclusive solution for the highly efficient synthesis of first-strand cDNA from mRNA or total RNA templates. Powered by our ultra-fast, highly processive mutant enzyme, use this kit to power through long transcripts and accelerate your workflows with speeds up to 100-fold faster than legacy standards!

DBscript 2.0 Reverse Transcriptase

DBscript 2.0 is an ultra-sensitive, thermostable M-MLV mutant engineered for highly efficient cDNA synthesis. Operating up to ~10-fold faster with exceptional thermostability, it is the optimal choice for 1-step RT-PCR, low copy number targets, and advanced diagnostic applications.

DBdirect™ RT-PCR Probe Mix SuperSens 2.0

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR SYBR Mix SuperSens 2.0

Next-generation of DB RT-PCR SYBR Mix SuperSens is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR SYBR Mix SuperSens 2.0 UDG

New-generation of DB RT-PCR SYBR Mix SuperSens UDG is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DBdirect™ RT-PCR SYBR Mix SuperSens 2.0 UDG

New generation of DBdirect™ RT-PCR SYBR 2.0 UDG mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

DBdirect™ RT-PCR SYBR Mix SuperSens 2.0

New generation of DBdirect™ RT-PCR SYBR 2.0 mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

DB RT-PCR Probe Mix SuperSens 2.0 UDG

Next-generation of DB RT-PCR Probe Mix SuperSens UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR Probe Mix SuperSens 2.0

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB RT-PCR Probe Mix SuperSens 2.0 dUTP

DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

DB Ultima H RNase Inhibitor

Unique approach to the RNase inhibition! DB Ultima H is highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market!

DIANA Biotechnologies | OEM Partnership

Looking for an OEM partner?

Contact our OEM Team

[email protected]