DB Ultima H RNase Inhibitor Lyo-Ready: Ultimate protection

DB Ultima H RNase Inhibitor: Absolute RNA Protection in Lyo-Ready Variant

(DB-1307)

RNase inhibitors are the only way to protect RNA in native samples. However, all protein inhibitors are highly unstable, while synthetic inhibitors have low potency and low assay compatibility. As a result, none of them reliably protects RNA.

That is why we developed DB Ultima H RNase inhibitor – a breakthrough, highly engineered human RNase inhibitor with the highest potency and unprecedented stability. It is fully oxidatively resistant and does not need reducing agents and as a result, it is stable and active across all relevant assay and storage conditions. Moreover, it is thermostable and can be kept at room temperature. It is the most potent inhibitor of human RNases and is the best option for protecting RNA in human samples.

It potently inhibits also other eukaryotic RNases and is effective against E.coli RNases. It outperforms other RNase inhibitors in RT-PCR, direct RT-PCR, cDNA synthesis, RNA-seq library preparation, in vitro transcription and other applications!

DB Ultima H RNase Inhibitor is now available in a Lyo-Ready formulation for seamless integration into lyophilized RT-PCR and diagnostic reagent mixes.

See the complete info in our DB Ultima H RNase Inhibitor leaflet!

Package size

Key Parameters: How does DB Ultima H improve your RNA protection?

Thermal Stability: The only protein inhibitor stable at or above 55°C.

High Potency: Highest possible inhibition of human RNases. Inhibits also other eukaryotic and E.coli RNases.

Extraordinary Resilience: Stable for months at room temperature. Fully active without DTT, cannot be oxidized. Compatible with the widest range of buffers & pH. Survives multiple freeze/thaw cycles.

Direct RT-PCR ready: Even higher activity than human RNase inhibitor in human tissues. Higher temperature ensure increased yields and sensitivity.

Lyo-Ready: Glycerol-free and detergent-free RNase inhibitor formulation designed for incorporation into lyophilized molecular biology and diagnostic reagent mixes.

Stop relying on unstable DTT to protect your RNA. DB Ultima H is the only inhibitor fully active without it.

DB Ultima H is the only oxidatively resistant inhibitor:Standard inhibitors on the market are very limited in oxidative resistance. This leaves the DB Ultima H the only truly oxidative resistant inhibitor even with 2% hydrogen peroxide use with no DTT. Numbers represent the same order of RNase inhibitors as it is in the first graph.

DB Ultima H stays fully active through freeze/thaw cycles that completely destroy standard inhibitors.

Freeze/thaw cycle stability:Left: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1mM DTT after a single freeze/thaw cycle at -20℃. Right: Residual RNase inhibitor activity in RT-PCR buffer with fresh 1 mM DTT after 24 hours on wet ice.

Maximize your RT-PCR sensitivity and yields: DB Ultima H stays fully active at 55°C, a temperature that completely destroys standard inhibitors.

Residual RNase Inhibitor activity after 5 min incubation at 55 ℃ in optimal buffer :DB Ultima H will enable RT-PCR in direct RT-qPCR at higher temperature; increasing yields and sensitivity 55°C is the optimal temperature for mutated M-MLV reverse transcriptase and optimal for UDG system.

Applications: DB Ultima H ensures RNA protection with no enzyme interference

Single-Cell Profiling & In Vitro Assays: Full RNA integrity guaranteed for prolonged in vitro protocols and single-cell expression profiling with zero enzyme interference.

RNA Structural & Functional Studies: Work across broad pH ranges and without DTT to establish perfect native conditions, completely free from the risk of sample degradation.

Direct RT-PCR from Human Samples: Bypass purification and amplify directly from crude lysates. Exceptional potency and oxidative resistance neutralize aggressive, RNase-rich environments in complex human tissues.

cDNA Synthesis & Cloning: Guarantee full-length transcripts. Safely melt complex secondary RNA structures at 55°C to maximize cDNA synthesis yields, without compromising downstream enzymatic steps.

Pricing

Cat. No.	Quantity	Lead Time
DB Ultima H RNase Inhibitor Lyo-Ready (DB-1307)	10 kU	In Stock
DB Ultima H RNase Inhibitor Lyo-Ready (DB-1307)	40 kU	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount required to inhibit 5 ng of RNase A activity by 50 %, using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate. Activity is measured in 100 mM Tris acetate pH 6.5, 0.5 mM EDTA buffer, at 25 °C	0.5 mM EDTA 20 mM HEPES pH = 7.5, 50 mM KCl, 8 mM DTT	Activity of the RNase inhibitor. Purity ≥ 90%, determined by SDS-PAGE. Absence of contaminating nucleases, including DNase, RNase, latent RNase, and endonuclease activity. Absence of E. coli genomic DNA.

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount required to inhibit 5 ng of RNase A activity by 50 %, using cytidine 2′,3′-cyclic monophosphate (cCMP) as the substrate. Activity is measured in 100 mM Tris acetate pH 6.5, 0.5 mM EDTA buffer, at 25 °C

0.5 mM EDTA

20 mM HEPES pH = 7.5,

50 mM KCl, 8 mM DTT

Activity of the RNase inhibitor.

Purity ≥ 90%, determined by SDS-PAGE.

Absence of contaminating nucleases, including DNase, RNase, latent RNase, and endonuclease activity.

Absence of E. coli genomic DNA.

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DB Ultima H RNase Inhibitor

Unique approach to the RNase inhibition! DB Ultima H is highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market!

Let's take your PCR to the next level!

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