DBdirect PCR Gel Mix SuperSens (DB-1275): Enhanced accuracy

DBdirect^™ PCR Gel Mix SuperSens

(DB-1275)

Introducing DBdirect™ PCR Gel Mix SuperSens, a ready-to-use solution featuring blue and yellow dyes for monitoring migration during electrophoresis and an additive for direct loading onto agarose gel.

Our unique DBdirect™ technology, enhanced with SuperSens, eliminates the need for DNA extraction, ensuring faster, more streamlined workflows with greater accuracy and sensitivity.

Package size

Key Parameters: Gel electrophoresis PCR with unique sesitivity

End-point PCR with antibody hot-start. Blue and yellow dyes to monitor migration process during electrophoresis.

Wide range of applications: qPCR from cDNA, genomic DNA (also for genotyping), plasmid DNA or preparation of DNA product for T/A cloning.

Direct detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media).

Suitable for single-cell detection. Certain cell types require a separate preincubation step in our Lysis Buffer A (DB-1281; feel free to request it with your order).

Pricing

Cat. no.	Quantity	Price	Lead Time
DBdirect™PCR Gel Mix SuperSens (DB-1275)	100 rxns	48 EUR	In Stock
DBdirect™PCR Gel Mix SuperSens (DB-1275)	1000 rxns	410 EUR	In Stock
DBdirect™PCR Gel Mix SuperSens (DB-1275)	5000 rxns	1770 EUR	In Stock

Details

Key Protein	Hot-start	Content	QC Assays
Taq DNA Polymerase	Antibody-based	DBdirect™ PCR Gel Mix SuperSens (2x) PCR Grade Water	Functional test (PCR) E. coli gDNA absence DNase Activity absence

Key Protein

Hot-start

Content

QC Assays

Taq DNA Polymerase

Antibody-based

DBdirect™ PCR Gel Mix SuperSens (2x)

PCR Grade Water

Functional test (PCR)

E. coli gDNA absence

DNase Activity absence

DBdirect PCR Gel Mix: Effortless Genotyping from Mouse Tissues

Hair and Tail: Minimal Tissue input for Precise Analysis:DBdirect™ PCR Gel Mix streamlines genotyping by enabling isolation-free DNA amplification directly from mouse tail tips and hair follicles. With rapid lysis and no purification required, it saves time and resources while delivering reliable, high-sensitivity results. Perfect for high-throughput workflows, DBdirect™ ensures consistent performance with minimal tissue input!

No DNA Isolation: Sensitive PCR from Microorganisms and Cells with DBdirect Technology

Straightforward PCR Analysis with no DNA Isolation:Our DBdirect™ technology enables direct PCR from a wide range of biological samples without the need for DNA isolation. The key innovation lies in our unique PCR mix formulation, which facilitates direct lysis during the PCR reaction. Some sample types benefit from a preincubation step in DB Lysis Buffer A (DB-1281), followed by the addition of the lysate to the PCR mix. Here we compared the sensitivity of our PCR mixes to various cell types and viruses and determined the optimal DBdirect™ variant for each category. If you are using E.coli, use only 5-minute denaturation step.

Sensitivity of DBdirect™ PCR mixes across cell types and viruses

Organisms	DB-1275 with LBA	DB-1275 PCR Mix
Human Cells	+++	+++
Gram-negative bacteria (E. coli)	+++	+++
Gram-positive bacteria (Lactobacillus)	++	+++
Gram-positive bacteria (Rhodococcus)	++	++
Yeast (S. Cerevisiae)	+++	++
Non-enveloped viruses (ADV)	+++	+++
Enveloped viruses (HSV-1)	+++	+++

+++ = 1 - 40 cells/rxn | ++ = 100 - 4000 cells/rxn | + = 10 000 - 400 000 cells/rxn

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