(DB-1284)
DB Random Hexamers are a mixture of random 6-nucleotide sequences that anneal across the entire length of target RNA molecules, recommended for cDNA synthesis from total RNA or RNA without a poly(A) tail.
Efficiently primes mRNA, siRNA, tRNA and other RNA species, producing a cDNA pool that represents the whole original transcript population. Supplied in two ready-to-use concentrations so you can match primer strength to your RNA input and downstream needs.
Package size
Key Parameters: Same primer mix, two options.
Variant A: 1.25 nmol. Best for routine cDNA synthesis at the recommended 1.25 µM working concentration, pipette directly, no dilution needed, for most standard applications.
Variant B: 6.25 nmol. Best for higher RNA input (up to 5 µg) where maximizing cDNA yield matters, and for preparing your own dilution series to fine-tune primer concentration.
Total RNA input range: 10 pg-5 μg.
Coverage: efficient transcription across the entire length of the target RNA via annealing at multiple sites.
Compatibility: works with most reverse transcriptases; recommended for use with DBscript Reverse Transcriptase (DB-1276), DBscript Reverse Transcriptase Turbo (DB-1333) and DBscript Reverse Transcriptase 2.0 (DB-1332).
Applications: cDNA synthesis from total RNA, including mRNA, siRNA and tRNA; recommended for RNA without a poly(A) tail.
Pricing
Name (Cat. no.) | Quantity | Price | Lead Time |
|---|---|---|---|
DB Random Hexamers (DB-1284-1.25nmol) | 50 μL / 1.25 nmol | 5 EUR | In Stock |
DB Random Hexamers (DB-1284-6.25nmol) | 50 μL / 6.25 nmol | 15 EUR | In Stock |
Downloads
Document name | Download |
|---|---|
Details
Storage | Shelf Life | Shipment | Quality Control |
|---|---|---|---|
≤ -18 °C for long-term storage | 3 years | Ambient temperature, or on dry ice on request | Primer concentration measured by UV absorbance for each lot; cDNA synthesis verified by detection of a 100 bp product in two-step RT-PCR |
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