DBdirect™ RT-PCR Probe Mix [Discontinued]

(DB-1263)

This product has been replaced with new series of enhanced DBdirect™ RT-PCR Probe Mix SuperSens 2.0 and DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG.


Discover the ultimate solution for routine and high-throughput RNA target detection with our DBdirect™ RT-PCR Probe Mix, designed specifically for TaqMan probes. Our unique RT-PCR technology eliminates the need for RNA extraction, streamlining your workflow and saving valuable time.


Trusted in millions of COVID-19 diagnostic tests. With SuperSens technology, you can analyze multiple targets in one reaction with exceptional sensitivity.

Package size

Key Parameters: No need for RNA isolation with faster and more streamlined workflows

Aptamer hot-start

Various applications including qualitative and quantitative real-time RT-PCR from RNA. Analyze multiple targets simultaneously in one reaction

Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

Suitable for single-cell detection: No need for pre-amplification and RNA isolation. Ideal for rare cell samples.

Product info

Cat. no.

Quantity

Lead Time

DBdirect™ RT-PCR Probe Mix (DB-1263)

100 rxns

Discontinued

DBdirect™ RT-PCR Probe Mix (DB-1263)

1000 rxns

Discontinued

DBdirect™ RT-PCR Probe Mix (DB-1263)

5000 rxns

Discontinued

Details

Key Proteins

Hot-start

Content

QC Assays

Taq DNA Polymerase

Thermostable reverse transcriptase (mutated M-MLV)

RNase Inhibitors

Aptamer-based

Enhancer mix (4x)

DBdirect™ RT-PCR Probe Mix (4x)


PCR Grade Water

Functional test (RT-PCR)

E. coli gDNA absence

RNase and DNase absence

Activity measurement of key proteins

Sensitive single cell detection with DBdirect RT-PCR Probe Mix

Profile the transcripts of interest to determine cell differentiation, stemness, marker expression, mutations, and more with no need for pre-amplification and RNA isolation:The DBdirect™ technology in our RT-PCR mixes allows for direct lysis and qRT-PCR in a single well. The streamlined protocol requires no RNA isolation or pre-amplification. In this study we measured transcripts in FACS sorted single cells directly into the PCR mixes. The results demonstrate that DBdirect™ RT-PCR mixes are sensitive enough to allow transcriptional profiling of single cells. Human cells (CCRF-CEM) were FACS sorted directly into 384-well plates containing 10 µl of the RT-PCR mixes, and subjected to 1-step qRT-PCR. Two assays consisting of primers and probes for GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) and TBP (TATA-box-binding protein) were used for the RT-PCR probe mix. GAPDH is a high-abundance transcript with thousands copies per cell, TBP is a low-abundance transcript with tens of copies per cell based on the protein atlas cell line expression data (if we assume 10 million transcripts per cell). Histograms of Ct values for GAPDH and TBP (n = 60 wells per PCR mix) revealed approximately log-normal distributions, indicating reliable detection. Grey bars indicate wells with no Ct values (also attributable to FACS errors). Results show approximately log-normal distributions of the Ct values, suggesting a reliable detection.

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