DBdirect™ RT-PCR Probe Mix [Discontinued]

(DB-1267)

This product has been replaced with new series of enhanced DBdirect™ RT-PCR Probe Mix SuperSens 2.0 and DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG.


Discover the ideal solution for routine and high-throughput RNA target detection with DBdirect™ RT-PCR Probe Mix SuperSens, specifically designed for TaqMan probes. Save time by analyzing multiple targets simultaneously in one reaction and experience unmatched accuracy and sensitivity with our unique SuperSens technology.


Trusted in millions of COVID-19 diagnostic tests. With SuperSens technology, you can analyze multiple targets in one reaction with exceptional sensitivity.

Package size

Key Parameters: Revolutionize RNA Detection with DBdirect™ RT-PCR Probe Mix SuperSens

Antibody hot-start with no need for NA extraction

Various applications including qualitative and quantitative real-time RT-PCR from RNA

Detection from a wide range of sample types (human cells, bacteria, viruses, different biological matrices such as human serum, saliva, or cell cultivation media)

Analyze multiple targets simultaneously in one reaction. Suitable for single-cell detection. Learn more!

Product info

Cat. no.

Quantity

Lead Time

DBdirect™ RT-PCR Probe Mix SuperSens (DB-1267)

100 rxns

Discontinued

DBdirect™ RT-PCR Probe Mix SuperSens (DB-1267)

1000 rxns

Discontinued

DBdirect™ RT-PCR Probe Mix SuperSens (DB-1267)

5000 rxns

Discontinued

Details

Key Proteins

Hot-start

Content

QC Assays

Taq DNA Polymerase

Thermostable reverse transcriptase (mutated M-MLV)

RNase Inhibitors

Antibody-based

Enhancer mix SuperSens (4x)

DBdirect™ RT-PCR Probe Mix SuperSens (4x)

PCR Grade Water

Functional test (RT-PCR)

E. coli gDNA

RNase andDNase Activity measurement of key proteins

Sensitive single cell detection with DBdirect RT-PCR Probe Mix SuperSens

Profile the transcripts of interest to determine cell differentiation, stemness, marker expression, mutations, and more with no need for pre-amplification and RNA isolation:The DBdirect™ technology in our RT-PCR mixes allows for direct lysis and qRT-PCR in a single well. The streamlined protocol requires no RNA isolation or pre-amplification. In this study we measured transcripts in FACS sorted single cells directly into the PCR mixes. The results demonstrate that DBdirect™ RT-PCR mixes are sensitive enough to allow transcriptional profiling of single cells. Human cells (CCRF-CEM) were FACS sorted directly into 384-well plates containing 10 µl of the RT-PCR mixes, and subjected to 1-step qRT-PCR. Two assays consisting of primers and probes for GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) and TBP (TATA-box-binding protein) were used for the RT-PCR probe mix. GAPDH is a high-abundance transcript with thousands copies per cell, TBP is a low-abundance transcript with tens of copies per cell based on the protein atlas cell line expression data (if we assume 10 million transcripts per cell). Histograms of Ct values for GAPDH and TBP (n = 60 wells per PCR mix) revealed approximately log-normal distributions, indicating reliable detection. Grey bars indicate wells with no Ct values (also attributable to FACS errors). Results show approximately log-normal distributions of the Ct values, suggesting a reliable detection.

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New generation of DBdirect™ RT-PCR SYBR 2.0 UDG mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

Let's take your PCR to the next level!

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