AF 647 NHS Ester: Fluorescent Dye

(DB-410402)

AF 647 NHS Ester is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 647. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.


The NHS ester is an amine-reactive dye: It can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules and is commonly used for flow cytometry and fluorescent microscopy.

Package size

Key Parameters: Ideal for all kinds of labeling with lower costs

NHS ester: label amine groups in proteins, peptides or amino-modified oligos and get stable signal generation

Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling

Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 647 with friendly price.

Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 647

Pricing

Cat. no.

Quantity

Price

DB-410402-1MG

1 mg

105 EUR





DB-410402-5MG

5 mg

335 EUR





DB-410405-10MG

10 mg

610 EUR





DB-410402-25MG

25 mg

1095 EUR






DB-410402-50MG

50 mg

1415 EUR





DB-410402-100MG

100 mg

1980 EUR




General Information

Shipping

Storage

Quality Control

Room Temperature

Up to 24 months in -20 ℃

HPLC (98 %+ purity)

Optical Data of AF 647 NHS Ester

Name

Appearance

Excitation (nm)

Emission (nm)

Excitation coeficient (ε)

MW (g/mol)

Formula

AF 594 NHS Ester

Dark-blue crystals

650

671

105 000

1245.63



C57H92N6O16S4

AF 647 NHS Ester: Absorption and emission spectra

Absorption and emission spectra of AF 647:The absorption and emission spectra of AF 647 NHS Ester visualized. The absorption peak at 650 nm ensures efficient excitation, while the emission peak at 671 nm provides strong fluorescence for high-sensitivity detection. Thanks to its high photostability, excellent pH resistance, and superior brightness, AF 647 is ideal for flow cytometry, confocal microscopy, and labeling of cell cultures and tissues.

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Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.

AF 647 is a bright far-red-fluorescent dye that is structurally identical to Alexa Fluor 647. AF 647 dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.

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