(DB-410403)
AF 647 Carboxylic Acid is a bright red-fluorescent dye that is structurally identical to Alexa Fluor 647. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.
AF 647 Carboxilic Acid is a non-reacitve and can be used as a reference standard for AF 647 conjugates. The carboxylic acid group can also be utilized for the synthesis of activated esters—such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol), and STP (4-sulfo-2,3,5,6-tetrafluorophenol)—or modified with hydrazines, hydroxylamines, or amines in aqueous media using water-soluble carbodiimides. This allows conjugation to molecules containing amino groups, including proteins, antibodies, and peptides. Consequently, AF 647 carboxylic acid is commonly used during solid-phase peptide synthesis to modify peptides in situ in the presence of activating agents like HATU.
Package size
Key Parameters: Great brightness and pH stability with lower costs
Carboxylic Acid: Ideal as a reference standard or for further synthesis
Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling
Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 647 with friendly price.
Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 647
Pricing
Cat. no. | Quantity | Price |
|---|---|---|
DB-410403-1MG | 1 mg | 105 EUR |
DB-410403-5MG | 5 mg | 315 EUR |
DB-410403-10MG | 10 mg | 590 EUR |
DB-410404-25MG | 25 mg | 1025 EUR |
DB-410405-50MG | 50 mg | 1340 EUR |
DB-410406-100MG | 100 mg | 1980 EUR |
General Information
Shipping | Storage | Quality Control |
|---|---|---|
Room Temperature | Up to 24 months in -20 ℃ | HPLC (98 %+ purity) |
Optical Data of AF 647 Carboxylic Ester
Name | Appearance | Excitation (nm) | Emission (nm) | Excitation coeficient (ε) | MW (g/mol) | Formula |
|---|---|---|---|---|---|---|
AF 647 Carboxylic Acid | Golden Blue Solid | 650 | 671 | 191 800 | 1148.56 | C53H89N5O14S4 |
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AF 647
AF 647 is a bright far-red-fluorescent dye that is structurally identical to Alexa Fluor 647. AF 647 dye possesses high water-solubility and high fluorescence quantum yield; it is pH-insensitive over a wide molar range. Widely applied for imaging and flow cytometry.
AF 647 NHS Ester
AF 647 NHS Ester is a bright far-red-fluorescent dye that is structurally identical to Alexa Fluor 647 NHS Ester. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 647 with NHS Ester is widely applied for imaging and flow cytometry.
