(DB-410203)
AF 568 Carboxylic Acid is a bright green-fluorescent dye that is structurally identical to Alexa Fluor 568. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences.
Its hydrophilic nature makes it an ideal choice for labeling sensitive proteins. AF 568 is perfect for a variety of imaging applications, including confocal microscopy, flow cytometry (FACS), and labeling cell cultures and tissues.
The carboxylic acid group can also be utilized for the synthesis of activated esters—such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol), and STP (4-sulfo-2,3,5,6-tetrafluorophenol)—or modified with hydrazines, hydroxylamines, or amines in aqueous media using water-soluble carbodiimides. This allows conjugation to molecules containing amino groups, including proteins, antibodies, and peptides. Consequently, AF 488 carboxylic acid is commonly used during solid-phase peptide synthesis to modify peptides in situ in the presence of activating agents like HATU.
Package size
Key Parameters: Stable signal generation and pH stability with lower costs
Carboxylic group: Ideal as a reference standard or for further synthesis
Applications: Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry (FACS), cell cultures and tissue labeling
Lower Cost Alternative: Same Spectral Properties & Performance – Works exactly like Alexa Fluor 568 with friendly price.
Complete Compatibility – Can be used in any existing protocol that uses Alexa Fluor 568
General information
Cat. no. | Quantity | Price | Shipping | Storage | Quality Control |
|---|---|---|---|---|---|
DB-410203-1MG | 1 mg | 93 EUR | Room Temperature | Up to 24 months in -20 ℃ | HPLC (95 %+ purity) |
DB-410203-5MG | 5 mg | 243 EUR | |||
DB-410203-10MG | 10 mg | 410 EUR | |||
DB-410203-25MG | 25 mg | 712 EUR | |||
DB-410203-50MG | 50 mg | 1218 EUR | |||
DB-410203-100MG | 100 mg | 1567 EUR |
Optical Data of AF 568 Carboxylic Acid
Name | Appearance | Excitation (nm) | Emission (nm) | Excitation coeficient (ε) | MW (g/mol) | Formula |
|---|---|---|---|---|---|---|
AF-568 Carboxylic Acid | Dark Orange | 579 | 603 | 94 000 | 897.11 | C45H60N4O11S2 |
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Our AF fluorescent dyes offer exceptional brightness, photostability, and versatility for your research. With identical structure as Alexa Fluor dyes, they provide the same spectral properties, quality, and reliability for a wide range of fluorescence-based applications. Whether you need precise antibody labeling, confocal and super resolution microscopy or sensitive flow cytometry detection, our AF dyes ensure complete compatibility with existing protocols.
AF 568 NHS ester
AF 568-NHS Ester is a bright orange-fluorescent dye that is structurally identical to Alexa Fluor 568 with NHS Ester attached. With its high photostability and pH resistance, it has become one of the most widely used fluorophores in life sciences. AF 568-NHS Ester can be conjugated with proteins and peptides, it is recommended for stable signal generation in imaging, including Western Blotting, fluorescence microscopy, and flow cytometry.
