DBscript Turbo Reverse Transcriptase: Ultra-fast RT

DBscript Turbo Reverse Transcriptase: Ultra-fast thermostable M-MLV mutant

(DB-1333)

Supercharge your reverse transcription workflow with DBscript Turbo, an ultra-fast and highly thermostable M-MLV mutant designed for the most demanding applications. Engineered for unparalleled speed and processivity, it operates ~100-fold faster than its competitors while delivering superior sensitivity.

With optimum activity between 50-60°C and the resilience to perform RT steps at up to 70°C, DBscript Turbo is the ultimate solution for generating full-length cDNA libraries, tackling long transcripts, and rapidly detecting low copy number targets down to a single copy.

Download the PDF catalogue filled with case studies here!

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Key Parameters: The best solution for most applications

Unmatched Speed: Performs ~100-fold faster than SuperScript III or III Flash, offering the same rapid synthesis as SuperScript IV but with enhanced sensitivity.

Extreme Thermostability: Features an optimum activity range of 50-60°C and remains highly functional for RT steps at up to an impressive 70°C.

Highly Processive: The ideal enzyme for long transcripts, ultra-short protocols, and full-length cDNA library synthesis.

Maximum Sensitivity: Suitable for low copy number targets, ensuring reliable detection down to ~1 copy.

General info

Name (Cat. no.)	Quantity	Lead Time
DBscript Turbo Reverse Transcriptase (DB-1333)	2 kU	In Stock
DBscript Turbo Reverse Transcriptase (DB-1333)	10 kU	In Stock
DBscript Turbo Reverse Transcriptase (DB-1333)	40 kU	In Stock

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.	20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol	SDS-PAGE/purity Functional Assay (cDNA synthesis)

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into

100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

SDS-PAGE/purity

Functional Assay (cDNA synthesis)

Downloads

Document name	Download

DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

DBscripts yield more cDNA: Across all temperatures from bacterial ribosomal RNA

Temperature-Dependent cDNA Yield Comparison:DBscript 2.0 and DBscript Turbo consistently produced the highest cDNA yields from bacterial ribosomal RNA across all tested temperatures. While all reverse transcriptases showed limited activity after 1 minute, DBscript 2.0 and particularly DBscript Turbo already generated measurable amounts of cDNA, demonstrating superior performance under rapid reaction conditions. After 10 minutes, both enzymes achieved near-complete cDNA synthesis at elevated temperatures (60–65°C), substantially outperforming conventional reverse transcriptases from Thermo, Roche, NEB, and Takara. The ability of DBscript enzymes to maintain high activity at increased temperatures supports more efficient transcription of structured RNA templates, resulting in higher cDNA yields and improved reaction specificity.

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