(DB-1333)
Supercharge your reverse transcription workflow with DBscript Turbo, an ultra-fast and highly thermostable M-MLV mutant designed for the most demanding applications. Engineered for unparalleled speed and processivity, it operates ~100-fold faster than its competitors while delivering superior sensitivity.
With optimum activity between 50-60°C and the resilience to perform RT steps at up to 70°C, DBscript Turbo is the ultimate solution for generating full-length cDNA libraries, tackling long transcripts, and rapidly detecting low copy number targets down to a single copy.
Package size
Key Parameters: The best solution for most applications
Unmatched Speed: Performs ~100-fold faster than SuperScript III or III Flash, offering the same rapid synthesis as SuperScript IV but with enhanced sensitivity.
Extreme Thermostability: Features an optimum activity range of 50-60°C and remains highly functional for RT steps at up to an impressive 70°C.
Highly Processive: The ideal enzyme for long transcripts, ultra-short protocols, and full-length cDNA library synthesis.
Maximum Sensitivity: Suitable for low copy number targets, ensuring reliable detection down to ~1 copy.
General info
Name (Cat. no.) | Quantity | Lead Time |
|---|---|---|
DBscript Turbo Reverse Transcriptase (DB-1333) | 2 kU | In Stock |
DBscript Turbo Reverse Transcriptase (DB-1333) | 10 kU | In Stock |
DBscript Turbo Reverse Transcriptase (DB-1333) | 40 kU | In Stock |
Details
Source | Unit Definition | Storage Buffer | QC Assays |
|---|---|---|---|
Recombinant E. coli | One unit is defined as the amount of enzyme that catalyzes incorporation of 1 nmol dNTPs (detected with SYBR® Green I dye) into 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C. | 20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40, 50 % glycerol | SDS-PAGE/purity Functional Assay (cDNA synthesis) |
Downloads
Document name | Download |
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