DBscript Reverse Transcriptase: Excellent cDNA Synthesis in Lyo-Ready Variant!

(DB-1346)

DBscript Reverse Transcriptase ensures excellent cDNA synthesis from RNA templates in both one-step and two-step RT-PCR applications.


With a superfast protocol and reduced pipetting steps, it features an MMLV mutant with reduced RNase H activity for improved yield and longer cDNAs (> 9 kb). It supports reverse transcription at 50-60 °C, thanks to increased thermostability and a unique buffer formulation. Expressed as a recombinant protein in E. coli, it comes with a 5X RT buffer, including dNTPs and a separate DTT solution.


DBscript Reverse Transcriptase is now available in a Lyo-Ready formulation for seamless integration into lyophilized RT-PCR and diagnostic reagent mixes.

Package size

Key Parameters: Superior cDNA Synthesis

Superfast Protocol: Reduced pipetting steps for streamlined workflows

Wide range sensitivity: Analyze 1 μg to 1 pg amount of RNA with 2-step RT-PCR

High-Temperature Stability: Reverse transcription at 50-60 °C due to increased thermostability and unique buffer formulation

Complete Kit: Supplied with 5X RT buffer, including dNTPs and a separate DTT solution

Lyo-Ready: Glycerol-free and detergent-free RNase inhibitor formulation designed for incorporation into lyophilized molecular biology and diagnostic reagent mixes.

Pricing

Name (Cat. no.)

Quantity

Lead Time

DBscript Reverse Transcriptase Lyo-Ready (DB-1346)

2 kU

Upon Request

Details

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount of enzyme that catalyzes the incorporation of 1 nmol of dNTPs (detected with SYBR™ Green I dye) within 10 min at 37 °C into 100 nM extendable DNA-RNA heteroduplex hairpin.

100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.

20 mM Tris 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.01 % NP-40,

50 % glycerol

Activity: the enzyme activity is measured for each lot and determined according to the unit definition.

Purity: SDS-PAGE shows at least 90 % purity. Each lot is also assayed for the presence of RNase, DNase, and endonuclease activity. The amount of E. coli genomic DNA (gDNA) is also determined.

Functional test: reverse transcription of a 2 000 bp RNA fragment and following PCR in one-step RT PCR using dilution series of RNA is performed and evaluated.

Downloads

Document name

Download

DBscript protocol is faster than its direct competitors

Efficient Reverse Transcription with DBscript Reverse Transcription:DBscript cDNA Synthesis Kit, powered by our thermostable DBscript Reverse Transcriptase, offers the shortest protocol time among top competitors. Its streamlined workflow accelerates primer annealing, incubation, and cDNA synthesis, without compromising performance. For researchers demanding precision and efficiency, DBscript is the optimal choice, minimizing time while maximizing output. This kit exemplifies our commitment to advancing molecular biology with faster, reliable results.

DBscript shows superior performance in more challenginig conditions

DBscript shows superior performance in challenging conditions:DBscript and competitor's reverse transcriptases were evaluated in 1-step qRT-PCR with equal amounts of a synthetic RNA template. Reactions were conducted in 1x RT-buffer A with 1x DTT, Bovine RNAse inhibitor (0.1 U/µl, DB-1260) and DB AptaTaq DNA Polymerase (0.2 U/µl, DB-1277). The relative cDNA yield was calculated using the equation1/2^(Ct - Ct ref) , where highest-performing sample (DBscript) served as a reference. This reflects the amount of cDNA produced relative to the reference sample. Error bars indicate standard deviation from three technical replicates. Under a challenging conditions, such as the transcription of a 1 kb template with a 2 minute reverse transcription step at 60°C, DBscript demonstrated superior performance and consistently yielded higher amounts of cDNA compared to competitor enzymes.
DBscript is effective at increased reverse transcription temperatures:Here we evaluated the reverse transcription yield of DBscript in 1-step RT-qPCR. Equal amounts of synthetic RNA template were subjected to 1-step qRT-PCR in a range of temperatures (37 - 60°C) and two different RT intervals (2 and 10 minutes). Relative cDNA yield was calculated relative to the highest value Ctref (55°C at 10 minutes) using the equation 1/(2^(Ct-Ct ref)). Error bars indicate standard deviation from three technical replicates. Our results show DBscript is stable even in increased reverse transcription temperatures up to 60°C.

Order or request free sample

I agree to the Processing of Personal Data

I agree to the Terms and Conditions

Related products

Customers who explored this product also showed interest in:

DBscript cDNA Synthesis Kit is an optimized kit containing all components needed for efficient synthesis of first-strand complementary DNA (cDNA) from mRNA or total RNA templates. Use our extremely fast protocol for your prompt analysis!

Next-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New-generation of DBdirect™ RT-PCR Probe Mix SuperSens 2.0 UDG is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. Direct technology is designed to skip the NA extraction step. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New generation of DBdirect™ RT-PCR SYBR 2.0 mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

New generation of DBdirect™ RT-PCR SYBR 2.0 UDG mix with higher sensitivity and robustness. Contains SYBR® Green I dye for real-time quantification of RNA sequences and offers an ideal solution for routine and high-throughput RNA target detection. Ready to use mix with SuperSens technology for greater accuracy and sensitivity!

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Next-generation of DB RT-PCR Probe Mix SuperSens is designed specifically for use with TaqMan probes and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Next-generation of DB RT-PCR SYBR Mix SuperSens is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

New-generation of DB RT-PCR SYBR Mix SuperSens UDG is designed specifically for use with SYBR Green I Dye® and offer an ideal solution for routine and high-throughput RNA target detection. You’ll achieve a greater accuracy and sensitivity with our SuperSens technology!

Unique approach to the RNase inhibition! DB Ultima H is highly engineered RNase inhibitor of human origin with full oxidative resistance and unparalleled thermal stability. It outperforms all other RNase inhibitors on the market! Now available in lyo-ready version.

Let's take your PCR to the next level!

Write us

[email protected]