DBscript 2.0 Reverse Transcriptase Lyo-Ready

DBscript 2.0 Reverse Transcriptase: Ultra-sensitive M-MLV mutant in Lyo-Ready Variant

(DB-1347)

Upgrade your reverse transcription workflow with DBscript 2.0 Reverse Transcriptase, an advanced, ultra-sensitive M-MLV mutant. Engineered to outperform standard enzymes, DBscript 2.0 delivers ~10-fold faster reaction times and higher thermostability than every competitor on the market.

Whether you are performing routine syntheses or detecting low copy number targets down to ~1 copy, this robust enzyme ensures reliable, maximum-yield cDNA synthesis for all your critical 1-step RT-PCR and diagnostic applications.

Th DBscript 2.0 Reverse Transcriptase is now available in a Lyo-Ready formulation for seamless integration into lyophilized RT-PCR and other RNA related analysis.

Download the PDF catalogue filled with case studies here!

Package size

Key Parameters: The Ideal Solution for Routine Applications in Lyo-Ready Variant

Maximum Sensitivity: Specifically optimized for low copy number targets, successfully detecting down to ~1 copy.

Exceptional Speed: Performs ~10-fold faster than Superscript III or III Flash.

High Thermostability: Features an optimum activity range of 50-60°C and maintains functionality for RT steps at up to 65°C.

Uncompromised Yield: Consistently delivers the same or better cDNA yield when compared to Superscripts.

Lyo-Ready: Glycerol-free and detergent-free RNase inhibitor formulation designed for incorporation into lyophilized molecular biology and diagnostic reagent mixes.

General info

Name (Cat. no.)	Quantity	Lead Time
DBscript 2.0 Reverse Transcriptase Lyo-Ready (DB-1348)	2 kU	Upon Request

Details

Source	Unit Definition	Storage Buffer	QC Assays
Recombinant E. coli	One unit is defined as the amount of enzyme that catalyzes the incorporation of 1 nmol of dNTPs (detected with SYBR™ Green I dye) within 10 min at 37 °C into 100 nM extendable DNA-RNA heteroduplex hairpin. 100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.	20 mM Tris 7.5, 100 mM NaCl, 0.5 mM DTT, 0.1 mM EDTA, 0.01% NP-40	Activity: the enzyme activity is measured for each lot and determined according to the unit definition. Purity: SDS-PAGE shows at least 90 % purity. Functional test: One-step RT-PCR: RT-PCR of a 500 bp RNA fragment.

Source

Unit Definition

Storage Buffer

QC Assays

Recombinant E. coli

One unit is defined as the amount of enzyme that catalyzes the incorporation of 1 nmol of dNTPs (detected with SYBR™ Green I dye) within 10 min at 37 °C into 100 nM extendable DNA-RNA heteroduplex hairpin.

100 nM extendable DNA-RNA heteroduplex hairpin within 10 min at 37 °C.

20 mM Tris 7.5, 100 mM NaCl, 0.5 mM DTT, 0.1 mM EDTA, 0.01% NP-40

Activity: the enzyme activity is measured for each lot and determined according to the unit definition.

Purity: SDS-PAGE shows at least 90 % purity.

Functional test: One-step RT-PCR: RT-PCR of a 500 bp RNA fragment.

Downloads

Document name	Download

DBscript shows higher cDNA yield than its competitors

cDNA yield after 5 min preincubation at various temperatures:Comparison of approximate cDNA yield reflecting enzyme stability after a 5-minute preincubation at various temperatures (Reaction conditions: 10-minute synthesis step at 55°C; 0.3 U/µL enzyme concentration; 500-base transcript). DBscript 2.0: Demonstrates outstanding resilience, retaining full cDNA yield even after a stringent 60°C preincubation, enabling higher reaction temperatures to ensure vastly improved sensitivity. DBscript Turbo: Delivers extreme thermal endurance, easily surviving 60°C preincubation with maximum yield and allowing for RT steps at 50–60°C even for longer, complex transcripts.

DBscript 2.0 and DBscript Turbo: Significantly faster protocols

Accelerated cDNA Synthesis Speed Comparison:Comparison of approximate cDNA yield between DIANA DBscript and Thermo SuperScript™ enzymes across 1-minute and 10-minute reaction times. Ultra-Fast Synthesis (1 min): In a rapid 1-minute reaction, DBscript 2.0 and DBscript Turbo operate >10x and >100x faster, respectively, than baseline SuperScript™ III, generating substantial yields before standard enzymes register significant activity. Maximum Yield (10 min): After a standard 10-minute incubation, the engineered DIANA mutants demonstrate profound dominance at elevated temperatures. At 60°C, both DBscript 2.0 and DBscript Turbo reach near-maximum yields (up to ~100%), drastically outperforming SuperScript™ IV and SuperScript™ III Flash.

DBscript 2.0 and Turbo yield more cDNA: Across all temperatures from bacterial ribosomal RNA

Temperature-Dependent cDNA Yield Comparison:DBscript 2.0 and DBscript Turbo consistently produced the highest cDNA yields from bacterial ribosomal RNA across all tested temperatures. While all reverse transcriptases showed limited activity after 1 minute, DBscript 2.0 and particularly DBscript Turbo already generated measurable amounts of cDNA, demonstrating superior performance under rapid reaction conditions. After 10 minutes, both enzymes achieved near-complete cDNA synthesis at elevated temperatures (60–65°C), substantially outperforming conventional reverse transcriptases from Thermo, Roche, NEB, and Takara. The ability of DBscript enzymes to maintain high activity at increased temperatures supports more efficient transcription of structured RNA templates, resulting in higher cDNA yields and improved reaction specificity.

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